A clinical model of dermal wound angiogenesis

Albert Lockhart, Rod D. Braun, Daohai Yu, Joel R. Ross, Mark W. Dewhirst, Bruce Klitzman, Fan Yuan, James M Grichnik, Alan D. Proia, Delina A. Conway, Gretchen Mann, Herbert I. Hurwitz

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Full-thickness dermal biopsies were performed in healthy volunteers to establish the range of angiogenic responses in wound healing in a normal population. Four-millimeter punch biopsies were made in the forearms of 15 healthy volunteers. Each wound was evaluated microscopically 4-5 times per week for 2 weeks. A semiquantitative wound scoring system to evaluate the neovasculature at the wound periphery was investigated. A vascular score was calculated for each wound at each observation. Two independent observers analyzed the microscopic wound images using the scoring system. At the end of the 14-day period, repeat biopsies were performed on some of the volunteers, and the granulation tissue was stained with anti-CD31. The Kaplan-Meier method was used to estimate the distribution of the time to reach predetermined target average vascular scores. A mixed-effects regression model indicated that time, age, and observer were predictors for the average vascular score outcome. The pattern and time course for wound neovascularization was highly reproducible in this group of healthy volunteers, and the assay was feasible and well tolerated. This wound angiogenesis model may be useful for monitoring the effects of antiangiogenic agents on normal wound neovascularization.

Original languageEnglish
Pages (from-to)306-313
Number of pages8
JournalWound Repair and Regeneration
Volume11
Issue number4
DOIs
StatePublished - Jul 1 2003
Externally publishedYes

Fingerprint

Skin
Wounds and Injuries
Blood Vessels
Healthy Volunteers
Biopsy
Angiogenesis Inhibitors
Granulation Tissue
Forearm
Wound Healing
Volunteers
Observation
Population

ASJC Scopus subject areas

  • Dermatology
  • Surgery

Cite this

Lockhart, A., Braun, R. D., Yu, D., Ross, J. R., Dewhirst, M. W., Klitzman, B., ... Hurwitz, H. I. (2003). A clinical model of dermal wound angiogenesis. Wound Repair and Regeneration, 11(4), 306-313. https://doi.org/10.1046/j.1524-475X.2003.11411.x

A clinical model of dermal wound angiogenesis. / Lockhart, Albert; Braun, Rod D.; Yu, Daohai; Ross, Joel R.; Dewhirst, Mark W.; Klitzman, Bruce; Yuan, Fan; Grichnik, James M; Proia, Alan D.; Conway, Delina A.; Mann, Gretchen; Hurwitz, Herbert I.

In: Wound Repair and Regeneration, Vol. 11, No. 4, 01.07.2003, p. 306-313.

Research output: Contribution to journalArticle

Lockhart, A, Braun, RD, Yu, D, Ross, JR, Dewhirst, MW, Klitzman, B, Yuan, F, Grichnik, JM, Proia, AD, Conway, DA, Mann, G & Hurwitz, HI 2003, 'A clinical model of dermal wound angiogenesis', Wound Repair and Regeneration, vol. 11, no. 4, pp. 306-313. https://doi.org/10.1046/j.1524-475X.2003.11411.x
Lockhart A, Braun RD, Yu D, Ross JR, Dewhirst MW, Klitzman B et al. A clinical model of dermal wound angiogenesis. Wound Repair and Regeneration. 2003 Jul 1;11(4):306-313. https://doi.org/10.1046/j.1524-475X.2003.11411.x
Lockhart, Albert ; Braun, Rod D. ; Yu, Daohai ; Ross, Joel R. ; Dewhirst, Mark W. ; Klitzman, Bruce ; Yuan, Fan ; Grichnik, James M ; Proia, Alan D. ; Conway, Delina A. ; Mann, Gretchen ; Hurwitz, Herbert I. / A clinical model of dermal wound angiogenesis. In: Wound Repair and Regeneration. 2003 ; Vol. 11, No. 4. pp. 306-313.
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