A centrosomal antigen localized on intermediate filaments and mitotic spindle poles

Brigitte Buendia, Claude Antony, Fulvia Verde, Michel Bornens, Eric Karsenti

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

A monoclonal antibody (CTR2611) raised against centrosomes isolated from human lymphocytes (KE37) stains the pericentriolar material and intermediate filaments in the same cells. In MDCK cells, where most of the microtubules do not originate from the pericentriolar region during interphase, the antigen is distributed along intermediate filaments. At the onset of mitosis, a large fraction of the CTR2611 antigen associates with the minus-end domain of the microtubules of the mitotic spindle but not with the pericentriolar region itself. Treatment of mitotic MDCK cells with taxol leads to the assembly of many microtubule asters in the cytoplasm at the expense of the mitotic spindle. The CTR2611 antigen is present in the center of each of these asters. Similar asters can also be produced in vitro by adding taxol to concentrated Xenopus egg mitotic cytoplasm. Again, the antigen is found close to the center of the asters. These results suggest that CTR2611 antigen is associated with a material involved in microtubule nucleation or microtubule minus-end stabilization. The monoclonal antibody recognizes a 74×103 Mr polypeptide and other polypeptides at 120×103 Mr and 170×103 Mr. The 74×103 Mr. polypeptide is found in all species examined so far, suggesting that it contains a highly conserved epitope.

Original languageEnglish
Pages (from-to)259-271
Number of pages13
JournalJournal of Cell Science
Volume97
Issue number2
StatePublished - Oct 1 1990
Externally publishedYes

Fingerprint

Spindle Poles
Spindle Apparatus
Intermediate Filaments
Microtubules
Centrosome
Antigens
Madin Darby Canine Kidney Cells
Paclitaxel
Peptides
Cytoplasm
Monoclonal Antibodies
Interphase
Xenopus
Mitosis
Ovum
Epitopes
Coloring Agents
Lymphocytes

Keywords

  • Centrosome
  • Intermediate filaments
  • Mitotic spindle

ASJC Scopus subject areas

  • Cell Biology

Cite this

Buendia, B., Antony, C., Verde, F., Bornens, M., & Karsenti, E. (1990). A centrosomal antigen localized on intermediate filaments and mitotic spindle poles. Journal of Cell Science, 97(2), 259-271.

A centrosomal antigen localized on intermediate filaments and mitotic spindle poles. / Buendia, Brigitte; Antony, Claude; Verde, Fulvia; Bornens, Michel; Karsenti, Eric.

In: Journal of Cell Science, Vol. 97, No. 2, 01.10.1990, p. 259-271.

Research output: Contribution to journalArticle

Buendia, B, Antony, C, Verde, F, Bornens, M & Karsenti, E 1990, 'A centrosomal antigen localized on intermediate filaments and mitotic spindle poles', Journal of Cell Science, vol. 97, no. 2, pp. 259-271.
Buendia, Brigitte ; Antony, Claude ; Verde, Fulvia ; Bornens, Michel ; Karsenti, Eric. / A centrosomal antigen localized on intermediate filaments and mitotic spindle poles. In: Journal of Cell Science. 1990 ; Vol. 97, No. 2. pp. 259-271.
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AB - A monoclonal antibody (CTR2611) raised against centrosomes isolated from human lymphocytes (KE37) stains the pericentriolar material and intermediate filaments in the same cells. In MDCK cells, where most of the microtubules do not originate from the pericentriolar region during interphase, the antigen is distributed along intermediate filaments. At the onset of mitosis, a large fraction of the CTR2611 antigen associates with the minus-end domain of the microtubules of the mitotic spindle but not with the pericentriolar region itself. Treatment of mitotic MDCK cells with taxol leads to the assembly of many microtubule asters in the cytoplasm at the expense of the mitotic spindle. The CTR2611 antigen is present in the center of each of these asters. Similar asters can also be produced in vitro by adding taxol to concentrated Xenopus egg mitotic cytoplasm. Again, the antigen is found close to the center of the asters. These results suggest that CTR2611 antigen is associated with a material involved in microtubule nucleation or microtubule minus-end stabilization. The monoclonal antibody recognizes a 74×103 Mr polypeptide and other polypeptides at 120×103 Mr and 170×103 Mr. The 74×103 Mr. polypeptide is found in all species examined so far, suggesting that it contains a highly conserved epitope.

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