TY - JOUR
T1 - A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication
AU - Jones, Katherine A.
AU - Kadonaga, James T.
AU - Rosenfeld, Philip J.
AU - Kelly, Thomas J.
AU - Tjian, Robert
N1 - Funding Information:
We thank Barbara Graves and Steve McKnight for the r/r promoter mutants, James Shen and Pam Mellon for supplying a-globin promoter constructs, and Bobbi Johnson for maintaining HeLa cells. This work was funded by research grants from the National Cancer Institute to R. T and T J. K. K. J. was a recipient of a fellowship from the National Institutes of Health; J. T. K. is a Fellow of the Miller Institute for Basic Research in Science; and P J. R. is supported by Medical Scientist Training Program Grant GMO7309. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
PY - 1987/1/16
Y1 - 1987/1/16
N2 - Transcription factor CTF, which is responsible for selective recognition of eukaryotic promoters that contain the sequence CCAAT, was purified to apparent homogeneity by sequence-specific DNA affinity chro-matography. Binding sites for CTF in the human Haras and α-globin promoters were highly homologous to sequences recognized by nuclear factor I (NF-I), a cellular DNA-binding protein that is required for the initiation of adenovirus DNA replication in vitro. To determine the relationship between CTF and NF-I, we compared the biochemical properties of these two proteins. CTF and NF-I were found to be indistinguishable in polypeptide composition, DNA-binding properties, immunological cross-reactivity, and in vitro stimulation of DNA replication and transcription initiation. We conclude that CTF/NF-I can serve both as a transcription selectivity factor for RNA polymerase II and as an initiation factor for adenovirus DNA replication.
AB - Transcription factor CTF, which is responsible for selective recognition of eukaryotic promoters that contain the sequence CCAAT, was purified to apparent homogeneity by sequence-specific DNA affinity chro-matography. Binding sites for CTF in the human Haras and α-globin promoters were highly homologous to sequences recognized by nuclear factor I (NF-I), a cellular DNA-binding protein that is required for the initiation of adenovirus DNA replication in vitro. To determine the relationship between CTF and NF-I, we compared the biochemical properties of these two proteins. CTF and NF-I were found to be indistinguishable in polypeptide composition, DNA-binding properties, immunological cross-reactivity, and in vitro stimulation of DNA replication and transcription initiation. We conclude that CTF/NF-I can serve both as a transcription selectivity factor for RNA polymerase II and as an initiation factor for adenovirus DNA replication.
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U2 - 10.1016/0092-8674(87)90358-8
DO - 10.1016/0092-8674(87)90358-8
M3 - Article
C2 - 3024847
AN - SCOPUS:0023657398
VL - 48
SP - 79
EP - 89
JO - Cell
JF - Cell
SN - 0092-8674
IS - 1
ER -