A cell cycle-regulated inhibitor of cyclin-dependent kinases

Ludger Hengst, Vjekoslav Dulic, Joyce M. Slingerland, Emma Lees, Steven I. Reed

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275 Scopus citations


Cyclin-dependent kinases (Cdks) previously have been shown to drive the major cell cycle transitions in eukaryotic organisms ranging from yeast to humans. We report here the identification of a 28-kDa protein, p28(Ick) (inhibitor of cyclin-dependent kinase), that binds to and inhibits the kinase activity of preformed Cdk/cyclin complexes from human cells. p28 inhibitory activity fluctuates during the cell cycle with maximal levels in G1 and accumulates in G1- and G0-arrested cells. These results suggest that control of the G1/S transition may be influenced by a family of Cdk inhibitors that include p28(Ick) and the recently described inhibitors p21(Cip1/Waf1/Cap20) and p16(Ink4).

Original languageEnglish (US)
Pages (from-to)5291-5295
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number12
StatePublished - Jun 7 1994
Externally publishedYes

ASJC Scopus subject areas

  • General


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