A cell based screening approach for identifying protein degradation regulators

Scott Simanski, Marie E. Maloof, Trey K. Sato, Valerie Cavett, Jennifer Caldwell Busby, Nagi Ayad

Research output: Contribution to journalArticle

Abstract

Cellular transitions are achieved by the concerted actions of regulated degradation pathways. In the case of the cell cycle, ubiquitin mediated degradation ensures unidirectional transition from one phase to another. For instance, turnover of the cell cycle regulator cyclin B1 occurs after metaphase to induce mitotic exit. To better understand pathways controlling cyclin B1 turnover, the N-terminal domain of cyclin B1 was fused to luciferase to generate an N-cyclin B1-luciferase protein that can be used as a reporter for protein turnover. Prior studies demonstrated that cell-based screens using this reporter identified small molecules inhibiting the ubiquitin ligase controlling cyclin B1-turnover. Our group adapted this approach for the G2-M regulator Wee1 where a Wee1-luciferase construct was used to identify selective small molecules inhibiting an upstream kinase that controls Wee1 turnover. In the present study we present a screening approach where cell cycle regulators are fused to luciferase and overexpressed with cDNAs to identify specific regulators of protein turnover. We overexpressed approximately 14,000 cDNAs with the N-cyclin B1-luciferase fusion protein and determined its steady-state level relative to other luciferase fusion proteins. We identified the known APC/C regulator Cdh1 and the F-box protein Fbxl15 as specific modulators of N-cyclin B1-luciferase steady-state levels and turnover. Collectively, our studies suggest that analyzing the steady-state levels of luciferase fusion proteins in parallel facilitates identification of specific regulators of protein turnover.

Original languageEnglish (US)
Pages (from-to)940-946
Number of pages7
JournalCell Cycle
Volume16
Issue number10
DOIs
StatePublished - May 19 2017

Keywords

  • cell-based screening
  • degradation
  • luciferase
  • substrate
  • ubiquitin

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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  • Cite this

    Simanski, S., Maloof, M. E., Sato, T. K., Cavett, V., Caldwell Busby, J., & Ayad, N. (2017). A cell based screening approach for identifying protein degradation regulators. Cell Cycle, 16(10), 940-946. https://doi.org/10.1080/15384101.2017.1301333