A broad amplification pattern at 3q in squamous cell lung cancer - A fluorescence in situ hybridization study

Eeva Kettunen, Wa'El El-Rifai, Anna Maria Björkqvist, Henrik Wolff, Antti Karjalainen, Sisko Anttila, Karin Mattson, Kirsti Husgafvel-Pursiainen, Sakari Knuutila

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Abstract

Frequent DNA copy number gain at 3q, with minimal overlapping area at 3q24-qter, has previously been reported in squamous cell carcinoma of the lung (SQCC), implicating the importance of genes at 3q in the tumorigenesis of SQCC. To further characterize the gain of DNA sequences at 3q, we performed interphase fluorescence in situ hybridization (FISH) analysis on 16 paraffin-embedded SQCC tumor samples that had previously been studied by comparative genomic hybridization (CGH). Eleven yeast artificial chromosome (YAC) probes located at 3q25~q27 and a chromosome 3-specific centromeric probe were used in the analysis. All SQCC tumors showed increase in DNA sequence copy number with 9-11 probes. In 5 tumors (31%) the number of centromeric signals varied from 3 to 5 and the YAC/centromeric signal ratio was 1.0, suggesting that the increase in DNA sequence copy number at 3q in these cases resulted from polysomy of chromosome 3. In 11 tumors (69%), the YAC/centromeric signal ratio varied between 1.5 and 4.7, indicating that the increase in DNA sequence copy number was due to intrachromosomal gain of DNA sequences at 3q. In each case, several YACs showed increased number of signals, demonstrating that the gained area was relatively large. Our findings therefore suggest that multiple genes located at 3q25~q27 are involved in the tumorigenesis of SQCC. Copyright (C) 2000 Elsevier Science Inc.

Original languageEnglish (US)
Pages (from-to)66-70
Number of pages5
JournalCancer Genetics and Cytogenetics
Volume117
Issue number1
DOIs
StatePublished - Feb 1 2000

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Kettunen, E., El-Rifai, WE., Björkqvist, A. M., Wolff, H., Karjalainen, A., Anttila, S., Mattson, K., Husgafvel-Pursiainen, K., & Knuutila, S. (2000). A broad amplification pattern at 3q in squamous cell lung cancer - A fluorescence in situ hybridization study. Cancer Genetics and Cytogenetics, 117(1), 66-70. https://doi.org/10.1016/S0165-4608(99)00146-6