A 3.9-centimorgan-resolution human single-nucleotide polymorphism linkage map and screening set

Tara C. Matise, Ravi Sachidanandam, Andrew G. Clark, Leonid Kruglyak, Ellen Wijsman, Jerzy Kakol, Steven Buyske, Buena Chui, Patrick Cohen, Claudia De Toma, Margaret Ehm, Stephen Glanowski, Chunsheng He, Jeremy Heil, Kyriacos Markianos, Ivy McMullen, Margaret A. Pericak-Vance, Arkadiy Silbergleit, Lincoln Stein, Michael WagnerAlexander F. Wilson, Jeffrey D. Winick, Emily S. Winn-Deen, Carl T. Yamashiro, Howard M. Cann, Eric Lai, Arthur L. Holden

Research output: Contribution to journalArticlepeer-review

99 Scopus citations

Abstract

Recent advances in technologies for high-throughout single-nucleotide polymorphism (SNP)-based genotyping have improved efficiency and cost so that it is now becoming reasonable to consider the use of SNPs for genomewide linkage analysis. However, a suitable screening set of SNPs and a corresponding linkage map have yet to be described. The SNP maps described here fill this void and provide a resource for fast genome scanning for disease genes. We have evaluated 6,297 SNPs in a diversity panel composed of European Americans, African Americans, and Asians. The markers were assessed for assay robustness, suitable allele frequencies, and informativeness of multi-SNP clusters. Individuals from 56 Centre d'Etude du Polymorphisme Humain pedigrees, with >770 potentially informative meioses altogether, were genotyped with a subset of 2,988 SNPs, for map construction. Extensive genotyping-error analysis was performed, and the resulting SNP linkage map has an average map resolution of 3.9 cM, with map positions containing either a single SNP or several tightly linked SNPs. The order of markers on this map compares favorably with several other linkage and physical maps. We compared map distances between the SNP linkage map and the interpolated SNP linkage map constructed by the deCode Genetics group. We also evaluated cM/Mb distance ratios in females and males, along each chromosome, showing broadly defined regions of increased and decreased rates of recombination. Evaluations indicate that this SNP screening set is more informative than the Marshfield Clinic's commonly used microsatellite-based screening set.

Original languageEnglish (US)
Pages (from-to)271-284
Number of pages14
JournalAmerican journal of human genetics
Volume73
Issue number2
DOIs
StatePublished - Aug 1 2003
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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