TY - JOUR
T1 - [58] Rabbit Liver tRNA Nucleotidyltransferase
AU - Deutscher, Murray P.
PY - 1974/1/1
Y1 - 1974/1/1
N2 - This chapter describes the procedures for the purification of the enzyme from Escherichia coli, and rabbit liver. This procedure yields a preparation of the enzyme from rabbit liver that is homogeneous and about 30,000-fold purified. The assay for tRNA nucleotidyltransferase measures the incorporation of radioactive, acid-soluble adenosine triphosphate (ATP) or cytosine triphosphate (CTP) into an acid-insoluble product. Purified preparations of rabbit liver tRNA nucleotidyltransferase contain a poly(C) polymerase activity as an integral part of the protein. This activity can attach long sequences of cytosine monophosphate (CMP) residues to any intact or partially degraded tRNA molecule or to rRNA. The rate of this activity under the usual assay conditions is only about 1% of the normal CMP incorporation and is generally not detected.
AB - This chapter describes the procedures for the purification of the enzyme from Escherichia coli, and rabbit liver. This procedure yields a preparation of the enzyme from rabbit liver that is homogeneous and about 30,000-fold purified. The assay for tRNA nucleotidyltransferase measures the incorporation of radioactive, acid-soluble adenosine triphosphate (ATP) or cytosine triphosphate (CTP) into an acid-insoluble product. Purified preparations of rabbit liver tRNA nucleotidyltransferase contain a poly(C) polymerase activity as an integral part of the protein. This activity can attach long sequences of cytosine monophosphate (CMP) residues to any intact or partially degraded tRNA molecule or to rRNA. The rate of this activity under the usual assay conditions is only about 1% of the normal CMP incorporation and is generally not detected.
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U2 - 10.1016/0076-6879(74)29063-3
DO - 10.1016/0076-6879(74)29063-3
M3 - Article
C2 - 4853999
AN - SCOPUS:0016007904
VL - 29
SP - 706
EP - 716
JO - Methods in Enzymology
JF - Methods in Enzymology
SN - 0076-6879
IS - C
ER -