5-Methyltryptamine decreases net accumulation of 32P into the polyphosphoinositides from [γ-32P]ATP in a cell-free system from blowfly salivary glands. Activation of breakdown of the newly synthesized [32P]polyphosphoinositides

I. Litosch; C. Calista; C. Wallis; J. N. Fain

5-Methyltryptamine decreases net accumulation of ³²P into the polyphosphoinositides from [γ-³²P]ATP in a cell-free system from blowfly salivary glands. Activation of breakdown of the newly synthesized [³²P]polyphosphoinositides

I. Litosch, C. Calista, C. Wallis, J. N. Fain

Research output: Contribution to journal › Article

Abstract

Incubation of blowfly salivary gland homogenates with 30 μM [γ-³²P]ATP resulted in a rapid, Mg²⁺-dependent, synthesis of [³²P]polyphosphoinositides and [³²P]phosphatidic acid. 5-Methyltryptamine, in the presence of 10 μM guanosine 5'-(3-O-thio)trisphosphate, reduced the net accumulation of ³²P label into phosphatidylinositol-4,5-P₂ and phosphatidylinositol-4-P by 35 and 20%, respectively. 5-Methyltryptamine did not affect synthesis of [³²P]phosphatidic acid. Phosphorylation of polyphosphoinositides was not affected by 5-methyltryptamine. In membranes labeled in vitro with [γ-³²P]ATP, 5-methyltryptamine stimulated a rapid breakdown of the [³²P]polyphosphoinositides. These results indicate that in blowfly salivary gland homogenates, hormone stimulates breakdown of the newly synthesized polyphosphoinositides. In the presence of hormone, the rate of polyphosphoinositide synthesis does not compensate for the rate of polyphosphoinositide degradation.

Original language	English
Pages (from-to)	638-643
Number of pages	6
Journal	Journal of Biological Chemistry
Volume	261
Issue number	2
State	Published - Jan 1 1986
Externally published	Yes

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Phosphatidylinositol Phosphates

Cell-Free System

Salivary Glands

Adenosine Triphosphate

Chemical activation

Phosphatidic Acids

Phosphatidylinositols

Hormones

Phosphorylation

Guanosine

5-methyltryptamine

Labels

Membranes

Degradation

ASJC Scopus subject areas

Biochemistry

Cite this

Litosch, I., Calista, C., Wallis, C., & Fain, J. N. (1986). 5-Methyltryptamine decreases net accumulation of ³²P into the polyphosphoinositides from [γ-³²P]ATP in a cell-free system from blowfly salivary glands. Activation of breakdown of the newly synthesized [³²P]polyphosphoinositides. Journal of Biological Chemistry, 261(2), 638-643.

5-Methyltryptamine decreases net accumulation of ³²P into the polyphosphoinositides from [γ-³²P]ATP in a cell-free system from blowfly salivary glands. Activation of breakdown of the newly synthesized [³²P]polyphosphoinositides. / Litosch, I.; Calista, C.; Wallis, C.; Fain, J. N.

In: Journal of Biological Chemistry, Vol. 261, No. 2, 01.01.1986, p. 638-643.

Research output: Contribution to journal › Article

Litosch, I, Calista, C, Wallis, C & Fain, JN 1986, '5-Methyltryptamine decreases net accumulation of ³²P into the polyphosphoinositides from [γ-³²P]ATP in a cell-free system from blowfly salivary glands. Activation of breakdown of the newly synthesized [³²P]polyphosphoinositides', Journal of Biological Chemistry, vol. 261, no. 2, pp. 638-643.

Litosch I, Calista C, Wallis C, Fain JN. 5-Methyltryptamine decreases net accumulation of ³²P into the polyphosphoinositides from [γ-³²P]ATP in a cell-free system from blowfly salivary glands. Activation of breakdown of the newly synthesized [³²P]polyphosphoinositides. Journal of Biological Chemistry. 1986 Jan 1;261(2):638-643.

Litosch, I. ; Calista, C. ; Wallis, C. ; Fain, J. N. / 5-Methyltryptamine decreases net accumulation of ³²P into the polyphosphoinositides from [γ-³²P]ATP in a cell-free system from blowfly salivary glands. Activation of breakdown of the newly synthesized [³²P]polyphosphoinositides. In: Journal of Biological Chemistry. 1986 ; Vol. 261, No. 2. pp. 638-643.

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abstract = "Incubation of blowfly salivary gland homogenates with 30 μM [γ-32P]ATP resulted in a rapid, Mg2+-dependent, synthesis of [32P]polyphosphoinositides and [32P]phosphatidic acid. 5-Methyltryptamine, in the presence of 10 μM guanosine 5'-(3-O-thio)trisphosphate, reduced the net accumulation of 32P label into phosphatidylinositol-4,5-P2 and phosphatidylinositol-4-P by 35 and 20{\%}, respectively. 5-Methyltryptamine did not affect synthesis of [32P]phosphatidic acid. Phosphorylation of polyphosphoinositides was not affected by 5-methyltryptamine. In membranes labeled in vitro with [γ-32P]ATP, 5-methyltryptamine stimulated a rapid breakdown of the [32P]polyphosphoinositides. These results indicate that in blowfly salivary gland homogenates, hormone stimulates breakdown of the newly synthesized polyphosphoinositides. In the presence of hormone, the rate of polyphosphoinositide synthesis does not compensate for the rate of polyphosphoinositide degradation.",

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N2 - Incubation of blowfly salivary gland homogenates with 30 μM [γ-32P]ATP resulted in a rapid, Mg2+-dependent, synthesis of [32P]polyphosphoinositides and [32P]phosphatidic acid. 5-Methyltryptamine, in the presence of 10 μM guanosine 5'-(3-O-thio)trisphosphate, reduced the net accumulation of 32P label into phosphatidylinositol-4,5-P2 and phosphatidylinositol-4-P by 35 and 20%, respectively. 5-Methyltryptamine did not affect synthesis of [32P]phosphatidic acid. Phosphorylation of polyphosphoinositides was not affected by 5-methyltryptamine. In membranes labeled in vitro with [γ-32P]ATP, 5-methyltryptamine stimulated a rapid breakdown of the [32P]polyphosphoinositides. These results indicate that in blowfly salivary gland homogenates, hormone stimulates breakdown of the newly synthesized polyphosphoinositides. In the presence of hormone, the rate of polyphosphoinositide synthesis does not compensate for the rate of polyphosphoinositide degradation.

AB - Incubation of blowfly salivary gland homogenates with 30 μM [γ-32P]ATP resulted in a rapid, Mg2+-dependent, synthesis of [32P]polyphosphoinositides and [32P]phosphatidic acid. 5-Methyltryptamine, in the presence of 10 μM guanosine 5'-(3-O-thio)trisphosphate, reduced the net accumulation of 32P label into phosphatidylinositol-4,5-P2 and phosphatidylinositol-4-P by 35 and 20%, respectively. 5-Methyltryptamine did not affect synthesis of [32P]phosphatidic acid. Phosphorylation of polyphosphoinositides was not affected by 5-methyltryptamine. In membranes labeled in vitro with [γ-32P]ATP, 5-methyltryptamine stimulated a rapid breakdown of the [32P]polyphosphoinositides. These results indicate that in blowfly salivary gland homogenates, hormone stimulates breakdown of the newly synthesized polyphosphoinositides. In the presence of hormone, the rate of polyphosphoinositide synthesis does not compensate for the rate of polyphosphoinositide degradation.

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