5-Methyltryptamine decreases net accumulation of 32P into the polyphosphoinositides from [γ-32P]ATP in a cell-free system from blowfly salivary glands. Activation of breakdown of the newly synthesized [32P]polyphosphoinositides

I. Litosch, C. Calista, C. Wallis, J. N. Fain

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Incubation of blowfly salivary gland homogenates with 30 μM [γ-32P]ATP resulted in a rapid, Mg2+-dependent, synthesis of [32P]polyphosphoinositides and [32P]phosphatidic acid. 5-Methyltryptamine, in the presence of 10 μM guanosine 5'-(3-O-thio)trisphosphate, reduced the net accumulation of 32P label into phosphatidylinositol-4,5-P2 and phosphatidylinositol-4-P by 35 and 20%, respectively. 5-Methyltryptamine did not affect synthesis of [32P]phosphatidic acid. Phosphorylation of polyphosphoinositides was not affected by 5-methyltryptamine. In membranes labeled in vitro with [γ-32P]ATP, 5-methyltryptamine stimulated a rapid breakdown of the [32P]polyphosphoinositides. These results indicate that in blowfly salivary gland homogenates, hormone stimulates breakdown of the newly synthesized polyphosphoinositides. In the presence of hormone, the rate of polyphosphoinositide synthesis does not compensate for the rate of polyphosphoinositide degradation.

Original languageEnglish
Pages (from-to)638-643
Number of pages6
JournalJournal of Biological Chemistry
Volume261
Issue number2
StatePublished - Jan 1 1986
Externally publishedYes

Fingerprint

Phosphatidylinositol Phosphates
Cell-Free System
Salivary Glands
Adenosine Triphosphate
Chemical activation
Phosphatidic Acids
Phosphatidylinositols
Hormones
Phosphorylation
Guanosine
5-methyltryptamine
Labels
Membranes
Degradation

ASJC Scopus subject areas

  • Biochemistry

Cite this

@article{674cb724b5414eb3869a5781aabbd5a3,
title = "5-Methyltryptamine decreases net accumulation of 32P into the polyphosphoinositides from [γ-32P]ATP in a cell-free system from blowfly salivary glands. Activation of breakdown of the newly synthesized [32P]polyphosphoinositides",
abstract = "Incubation of blowfly salivary gland homogenates with 30 μM [γ-32P]ATP resulted in a rapid, Mg2+-dependent, synthesis of [32P]polyphosphoinositides and [32P]phosphatidic acid. 5-Methyltryptamine, in the presence of 10 μM guanosine 5'-(3-O-thio)trisphosphate, reduced the net accumulation of 32P label into phosphatidylinositol-4,5-P2 and phosphatidylinositol-4-P by 35 and 20{\%}, respectively. 5-Methyltryptamine did not affect synthesis of [32P]phosphatidic acid. Phosphorylation of polyphosphoinositides was not affected by 5-methyltryptamine. In membranes labeled in vitro with [γ-32P]ATP, 5-methyltryptamine stimulated a rapid breakdown of the [32P]polyphosphoinositides. These results indicate that in blowfly salivary gland homogenates, hormone stimulates breakdown of the newly synthesized polyphosphoinositides. In the presence of hormone, the rate of polyphosphoinositide synthesis does not compensate for the rate of polyphosphoinositide degradation.",
author = "I. Litosch and C. Calista and C. Wallis and Fain, {J. N.}",
year = "1986",
month = "1",
day = "1",
language = "English",
volume = "261",
pages = "638--643",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "2",

}

TY - JOUR

T1 - 5-Methyltryptamine decreases net accumulation of 32P into the polyphosphoinositides from [γ-32P]ATP in a cell-free system from blowfly salivary glands. Activation of breakdown of the newly synthesized [32P]polyphosphoinositides

AU - Litosch, I.

AU - Calista, C.

AU - Wallis, C.

AU - Fain, J. N.

PY - 1986/1/1

Y1 - 1986/1/1

N2 - Incubation of blowfly salivary gland homogenates with 30 μM [γ-32P]ATP resulted in a rapid, Mg2+-dependent, synthesis of [32P]polyphosphoinositides and [32P]phosphatidic acid. 5-Methyltryptamine, in the presence of 10 μM guanosine 5'-(3-O-thio)trisphosphate, reduced the net accumulation of 32P label into phosphatidylinositol-4,5-P2 and phosphatidylinositol-4-P by 35 and 20%, respectively. 5-Methyltryptamine did not affect synthesis of [32P]phosphatidic acid. Phosphorylation of polyphosphoinositides was not affected by 5-methyltryptamine. In membranes labeled in vitro with [γ-32P]ATP, 5-methyltryptamine stimulated a rapid breakdown of the [32P]polyphosphoinositides. These results indicate that in blowfly salivary gland homogenates, hormone stimulates breakdown of the newly synthesized polyphosphoinositides. In the presence of hormone, the rate of polyphosphoinositide synthesis does not compensate for the rate of polyphosphoinositide degradation.

AB - Incubation of blowfly salivary gland homogenates with 30 μM [γ-32P]ATP resulted in a rapid, Mg2+-dependent, synthesis of [32P]polyphosphoinositides and [32P]phosphatidic acid. 5-Methyltryptamine, in the presence of 10 μM guanosine 5'-(3-O-thio)trisphosphate, reduced the net accumulation of 32P label into phosphatidylinositol-4,5-P2 and phosphatidylinositol-4-P by 35 and 20%, respectively. 5-Methyltryptamine did not affect synthesis of [32P]phosphatidic acid. Phosphorylation of polyphosphoinositides was not affected by 5-methyltryptamine. In membranes labeled in vitro with [γ-32P]ATP, 5-methyltryptamine stimulated a rapid breakdown of the [32P]polyphosphoinositides. These results indicate that in blowfly salivary gland homogenates, hormone stimulates breakdown of the newly synthesized polyphosphoinositides. In the presence of hormone, the rate of polyphosphoinositide synthesis does not compensate for the rate of polyphosphoinositide degradation.

UR - http://www.scopus.com/inward/record.url?scp=0022572312&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022572312&partnerID=8YFLogxK

M3 - Article

C2 - 3001073

AN - SCOPUS:0022572312

VL - 261

SP - 638

EP - 643

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 2

ER -