Both rachitic 1,25-dihydroxycholecalciferol-treated and normal chicks produced detectable quantities of 25-hydroxycholecalciferol-26,23-lactone, although renal homogenates from rachitic chicks failed to produce this compound. The addition of either 25-hydroxycholecalciferol or 25,26-dihydroxycholecalciferol to the renal homogenates led to the production of the lactone, although more lactone resulted when 25,26-dihydroxycholecalciferol was used. When tritiated 25-hydroxycholecalciferol was added to rachitic 1,25-dihydroxycholecalciferol-treated or normal chick renal homogenates, an unidentified tritiated vitamin D metabolite distinct from 25-hydroxycholecalciferol-26,23-lactone was produced.
ASJC Scopus subject areas
- Molecular Biology