Abstract
In vitro culture of H9 human lymphoid cells in the presence of 5.0 μM dideoxycytidine (ddC), for about 40-45 days, selected cells (H9-ddC cells), which were resistant to the drug and cross-resistant to AZT (zidovudine) and 5-fluoro-2′-deoxyuridine (FdUR). The major mechanism of cross-resistance to AZT and FdUR in these cells was low cellular activity of thymidine kinase (TK). To explore molecular mechanisms of the reduced TK activity in H9-ddC cells, the mRNA expression of TK1 and TK2 and western blot analysis of TK1 protein were performed. RT-PCR analysis revealed that in H9-ddC cells the expression of both TK1 and TK2 mRNA was reduced to 27.1% and 79.4%, respectively. The reduced TK1 gene expression was confirmed by an absence of a detectable TK1 protein band in western blot of H9-ddC cells. These results demonstrate that long-term treatment of H9 cells in the presence of ddC down-regulated TK1 and TK2 gene expression and reduced the expression and activity of TK in the resistant cells.
Original language | English (US) |
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Pages (from-to) | 835-842 |
Number of pages | 8 |
Journal | Life Sciences |
Volume | 74 |
Issue number | 7 |
DOIs | |
State | Published - Jan 2 2004 |
Keywords
- Cross-resistance
- Deoxycytidine kinase
- Dideoxycytidine
- Gene down regulation
- Thymidine kinase 2
- Thymidine kinase1
ASJC Scopus subject areas
- Pharmacology