17β2-Estradiol modifies diabetic wound healing by decreasing matrix metalloproteinase activity

David J. Pincus, Noor Kassira, Michael Gombosh, Mariana Berho, Marilyn K Glassberg Csete, Michael Karl, Sharon Elliot, Seth Thaller

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Postmenopausal women are more susceptible to poor wound healing. This phenomenon can be reversed by estrogen replacement therapy in non-diabetic individuals. Postmenopausal women with type 2 diabetes are more susceptible to wound healing complications, potentially secondary to an estrogen deficiency. Few studies have examined the mechanism of action and effects of estrogens on diabetic wound healing in females. It appears that multiple factors influence delayed wound healing among individuals with diabetes including: an imbalance in cytokines, growth factors, extracellular matrix (ECM) turnover, and oxidant stress (OS). Estrogens have been shown to regulate the expression of genes important for extracellular matrix turnover, including collagen and matrix metalloproteinases (MMP). Methods. For this reason, the effects of 17β2-estradiol (E2) on MMP-2, MMP-13, and MMP-14 and estrogen receptor alpha and beta (ER-α and -β) expression in the wound tissue of estrogen-deficient female mice with established type 2 diabetes mellitus (C57BL/6J-m Leprdb/2+) were studied. Results. Topical E2 upregulates ER in wound tissue thereby improving and accelerating diabetic wound healing in estrogen deficient mice. Conclusion. The mechanism appears to decrease MMP-2, MMP-13, and MMP-14 mediated tissue matrix destruction and increasing collagen content.

Original languageEnglish
Pages (from-to)171-178
Number of pages8
JournalWounds
Volume22
Issue number7
StatePublished - Jul 1 2010

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Matrix Metalloproteinases
Wound Healing
Estradiol
Estrogens
Matrix Metalloproteinase 13
Matrix Metalloproteinase 14
Matrix Metalloproteinase 2
Type 2 Diabetes Mellitus
Extracellular Matrix
Collagen
Estrogen Receptor beta
Estrogen Replacement Therapy
Estrogen Receptor alpha
Wounds and Injuries
Oxidants
Intercellular Signaling Peptides and Proteins
Up-Regulation
Cytokines
Gene Expression

ASJC Scopus subject areas

  • Surgery
  • Medical–Surgical

Cite this

17β2-Estradiol modifies diabetic wound healing by decreasing matrix metalloproteinase activity. / Pincus, David J.; Kassira, Noor; Gombosh, Michael; Berho, Mariana; Glassberg Csete, Marilyn K; Karl, Michael; Elliot, Sharon; Thaller, Seth.

In: Wounds, Vol. 22, No. 7, 01.07.2010, p. 171-178.

Research output: Contribution to journalArticle

Pincus DJ, Kassira N, Gombosh M, Berho M, Glassberg Csete MK, Karl M et al. 17β2-Estradiol modifies diabetic wound healing by decreasing matrix metalloproteinase activity. Wounds. 2010 Jul 1;22(7):171-178.
Pincus, David J. ; Kassira, Noor ; Gombosh, Michael ; Berho, Mariana ; Glassberg Csete, Marilyn K ; Karl, Michael ; Elliot, Sharon ; Thaller, Seth. / 17β2-Estradiol modifies diabetic wound healing by decreasing matrix metalloproteinase activity. In: Wounds. 2010 ; Vol. 22, No. 7. pp. 171-178.
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