17β-Estradiol regulates and v-Ha-ras transfection constitutively enhances MCF7 breast cancer cell interactions with basement membrane

A. Albini, J. Graf, G. T. Kitten, H. K. Kleinman, G. R. Martin, A. Veillette, Marc E Lippman

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

The interaction of a line of human breast carcinoma cells (MCF7) with basement membrane components, particularly laminin, was altered by exposure of the cells to estrogen as well as by transfection of the cells with the v-Ha-ras oncogene. In both cases, the cells show a greater ability to attach to a laminin substrate, to migrate to laminin, to grow in the presence of a basement membrane matrix, and to cross barriers of reconstituted basement membrane. These responses were associated with an increase in the expression of laminin receptors. It is postulated that the increase in the invasive behavior of the cells treated with estrogen or transfected with v-Ha-ras is related to the increased number of laminin receptors and their interaction with laminin. Estrogen has no discernible effect on the v-Ha-ras transfected cells. It appears that in the MCF7 cells, the malignant phenotype is under hormonal control and that this control is bypassed after v-Ha-ras transfection.

Original languageEnglish
Pages (from-to)8182-8186
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number21
DOIs
StatePublished - Dec 1 1986
Externally publishedYes

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Basement Membrane
Cell Communication
Transfection
Laminin
Estradiol
Breast Neoplasms
Laminin Receptors
Estrogens
MCF-7 Cells
ras Genes
Phenotype

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

17β-Estradiol regulates and v-Ha-ras transfection constitutively enhances MCF7 breast cancer cell interactions with basement membrane. / Albini, A.; Graf, J.; Kitten, G. T.; Kleinman, H. K.; Martin, G. R.; Veillette, A.; Lippman, Marc E.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 21, 01.12.1986, p. 8182-8186.

Research output: Contribution to journalArticle

Albini, A. ; Graf, J. ; Kitten, G. T. ; Kleinman, H. K. ; Martin, G. R. ; Veillette, A. ; Lippman, Marc E. / 17β-Estradiol regulates and v-Ha-ras transfection constitutively enhances MCF7 breast cancer cell interactions with basement membrane. In: Proceedings of the National Academy of Sciences of the United States of America. 1986 ; Vol. 83, No. 21. pp. 8182-8186.
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