1,25-dihydroxyvitamin D3antagonizes interferon-γ- induced expression of class II major histocompatibility antigens on thyroid follicular and testicular leydig cells

Noriaki Tokuda, Tomiya Mano, Robert B. Levy

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Interferon-γ (IFNγ) induces production and expression of major histocompatibility complex class II molecules on both marrow-derived and nonbone marrow-derived cell types. 1,25-Dihydroxyvitamin D3 [1,25-(OH)2D3], a seco-steroid derived from vitamin D3, has previously been reported to enhance such expression alone or together with IFNγ on a number of monocyte/macrophage tumorigenic lines. In contrast, the present studies have found that 1,25-(OH)2D3 inhibited the ability of IFNγ to induce class II antigen expression on non-transformed rat thyroid follicular epithelial cells (FRTL-5) and mouse testicular Leydig cells (TM3). Although 1,25-(OH)2D3 inhibited the induction of both IA and IE class II locus products, IFNγ augmentation of class I major histocompatibility complex antigens was not affected. 1,24-(OH)2D3 and 24,25-(OH)2D3 also inhibited class II induction by IFNγ. Notably, the relative inhibitory ability of these compounds paralleled the strength of their binding affinities for the 1,25-(OH)2D3 receptor, indicating that this antagonistic effect probably requires receptor-ligand interaction. Other steroid hormones, such as hydrocortisone or testosterone, had no inhibitory effect on IFNγ-induced class II expression on Leydig cells. Additionally, the failure of indomethacin to reverse the effect of 1,25-(OH)2D3 and the finding that exogenous prostaglandin E2 did not inhibit class II induction in these cells indicated that prostaglandins are probably not responsible for this anti-IFNγ activity. In total, these results suggest that an endocrinological mediator is capable of inhibiting class II induction on resident endocrine tissue populations and, therefore, could help to diminish local CD4+ T-cell recognition of these cells.

Original languageEnglish (US)
Pages (from-to)1419-1427
Number of pages9
JournalEndocrinology
Volume127
Issue number3
DOIs
StatePublished - Jan 1 1990

ASJC Scopus subject areas

  • Endocrinology

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