1,25-dihydroxyvitamin D3 down-regulation of HLA-DR on human peripheral blood monocytes

N. Tokuda, N. Mizuki, M. Kasahara, R. B. Levy

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

The regulatory activity of 1,25-dihydoxyvitamin D3 (1,25-(OH)2D3) on human leucocyte antigen (HLA)-DR (MHC class II) antigen expression in monocytes from normal human peripheral blood was examined. Using forward light and side scatter by flow cytometry most cells within the discrete monocyte area expressed high levels of HLA-DR antigens following 4-day culture in medium alone (culture-enhanced HLA-DR) and expression was further up-regulated in the presence of interferon gamma (IFN-γ) (IFN-γ-enhanced HLA-DR). Treatment with 1,25-(OH)2D3 markedly inhibited both culture and IFN-γ-enhanced HLA-DR but not HLA-ABC (MHC class I). This 1,25-(OH)2D3 inhibition was as effective as PGE2 and hydrocortisone. To ascertain if HLA-DR was specifically down-regulated on monocytes, the effect of vitamin D3 analogues in CD33+ cells was examined. Incubation of the CD33+ cells with 1,25-(OH)2D3, 24-25-(OH)2D3 and 25-OHD3 resulted in dose-dependent inhibition of culture-enhanced HLA-DR parallelling the vitamin D3-receptor affinities of these compounds. Northern analysis also demonstrated that 1,25-(OH)2D3 treatment markedly decreased both expression of culture-enhanced and IFN-γ-enhanced HLA-DRβ chain messanger RNA (mRNA) in monocyte-enriched populations. In total, our findings are consistent with the proposal that vitamin D3 analogues can contribute to down-regulating immune responses as a consequence of inhibiting class II expression.

Original languageEnglish (US)
Pages (from-to)349-354
Number of pages6
JournalImmunology
Volume75
Issue number2
StatePublished - 1992

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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