Abstract
In Escherichia coli, χ is a recombination hotspot that stimulates RecBCD- dependent exchange at and to one side of itself. χ active is highest at χ and decreases with distance from χ. The decrease in χ activity may be a simple property of the physical distance over which χ can stimulate recombination. Alternatively, the decay in χ activity with distance may reflect the high likelihood that χ-stimulated recombination occurs in a single χ-proximal act, to the exclusion of additional χ-stimulated exchanges more distal to χ. To test the models, we determined if χ activity decreases as a function of physical distance (i.e., DNA base pairs) or genetic distance (homologous DNA base pairs). Our results indicate that χ activity decays as a function of genetic distance. In addition, we found that the sbcB gene product (exonuclease 1, a 3' → 5' ssDNA exonuclease) modulates the distance over which χ can act. In contrast, the recJ gene product (a 5' → 3' ssDNA exonuclease) does not alter the decay of χ activity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 805-812 |
| Number of pages | 8 |
| Journal | Genetics |
| Volume | 141 |
| Issue number | 3 |
| State | Published - Jan 1 1995 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
- Genetics(clinical)
Cite this
χ recombination activity in phage λ decays as a function of genetic distance. / Myers, Richard; Stahl, M. M.; Stahl, F. W.
In: Genetics, Vol. 141, No. 3, 01.01.1995, p. 805-812.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - χ recombination activity in phage λ decays as a function of genetic distance
AU - Myers, Richard
AU - Stahl, M. M.
AU - Stahl, F. W.
PY - 1995/1/1
Y1 - 1995/1/1
N2 - In Escherichia coli, χ is a recombination hotspot that stimulates RecBCD- dependent exchange at and to one side of itself. χ active is highest at χ and decreases with distance from χ. The decrease in χ activity may be a simple property of the physical distance over which χ can stimulate recombination. Alternatively, the decay in χ activity with distance may reflect the high likelihood that χ-stimulated recombination occurs in a single χ-proximal act, to the exclusion of additional χ-stimulated exchanges more distal to χ. To test the models, we determined if χ activity decreases as a function of physical distance (i.e., DNA base pairs) or genetic distance (homologous DNA base pairs). Our results indicate that χ activity decays as a function of genetic distance. In addition, we found that the sbcB gene product (exonuclease 1, a 3' → 5' ssDNA exonuclease) modulates the distance over which χ can act. In contrast, the recJ gene product (a 5' → 3' ssDNA exonuclease) does not alter the decay of χ activity.
AB - In Escherichia coli, χ is a recombination hotspot that stimulates RecBCD- dependent exchange at and to one side of itself. χ active is highest at χ and decreases with distance from χ. The decrease in χ activity may be a simple property of the physical distance over which χ can stimulate recombination. Alternatively, the decay in χ activity with distance may reflect the high likelihood that χ-stimulated recombination occurs in a single χ-proximal act, to the exclusion of additional χ-stimulated exchanges more distal to χ. To test the models, we determined if χ activity decreases as a function of physical distance (i.e., DNA base pairs) or genetic distance (homologous DNA base pairs). Our results indicate that χ activity decays as a function of genetic distance. In addition, we found that the sbcB gene product (exonuclease 1, a 3' → 5' ssDNA exonuclease) modulates the distance over which χ can act. In contrast, the recJ gene product (a 5' → 3' ssDNA exonuclease) does not alter the decay of χ activity.
UR - http://www.scopus.com/inward/record.url?scp=0028971616&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028971616&partnerID=8YFLogxK
M3 - Article
C2 - 8582627
AN - SCOPUS:0028971616
VL - 141
SP - 805
EP - 812
JO - Genetics
JF - Genetics
SN - 0016-6731
IS - 3
ER -