χ recombination activity in phage λ decays as a function of genetic distance

Richard Myers, M. M. Stahl, F. W. Stahl

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In Escherichia coli, χ is a recombination hotspot that stimulates RecBCD- dependent exchange at and to one side of itself. χ active is highest at χ and decreases with distance from χ. The decrease in χ activity may be a simple property of the physical distance over which χ can stimulate recombination. Alternatively, the decay in χ activity with distance may reflect the high likelihood that χ-stimulated recombination occurs in a single χ-proximal act, to the exclusion of additional χ-stimulated exchanges more distal to χ. To test the models, we determined if χ activity decreases as a function of physical distance (i.e., DNA base pairs) or genetic distance (homologous DNA base pairs). Our results indicate that χ activity decays as a function of genetic distance. In addition, we found that the sbcB gene product (exonuclease 1, a 3' → 5' ssDNA exonuclease) modulates the distance over which χ can act. In contrast, the recJ gene product (a 5' → 3' ssDNA exonuclease) does not alter the decay of χ activity.

Original languageEnglish (US)
Pages (from-to)805-812
Number of pages8
JournalGenetics
Volume141
Issue number3
StatePublished - Jan 1 1995
Externally publishedYes

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Bacteriophages
Genetic Recombination
Exonucleases
Base Pairing
DNA
Genes
Escherichia coli
exodeoxyribonuclease I

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

χ recombination activity in phage λ decays as a function of genetic distance. / Myers, Richard; Stahl, M. M.; Stahl, F. W.

In: Genetics, Vol. 141, No. 3, 01.01.1995, p. 805-812.

Research output: Contribution to journalArticle

Myers, R, Stahl, MM & Stahl, FW 1995, 'χ recombination activity in phage λ decays as a function of genetic distance', Genetics, vol. 141, no. 3, pp. 805-812.
Myers, Richard ; Stahl, M. M. ; Stahl, F. W. / χ recombination activity in phage λ decays as a function of genetic distance. In: Genetics. 1995 ; Vol. 141, No. 3. pp. 805-812.
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