χ and the RecBC D enzyme of Escherichia coli

Richard Myers, Franklin W. Stahl

Research output: Contribution to journalReview article

161 Citations (Scopus)

Abstract

The products of genes recB and recC are responsible for conjugal recombination and for the repair of chromosomal double chain breaks in Escherichia coli. The product of the recD gene, which combines with the RecB and RecC proteins to comprise the RecBCD enzyme, is not required for either recombination or repair. On the contrary, RecBCD enzyme is a potent exonuclease which inhibits recombination by destroying linear DNA. The RecD Ejection model supposes that RecBCD enzyme enters DNA at a double-chain end and travels destructively along the DNA until (typically) it encounters the recombination hotspot sequence χ. χ then alters the RecBCD enzyme by weakening the affinity of the RecD subunit for the RecBC heterodimer. With the loss of the RecD subunit from the enzyme, the resulting protein, RecBC(D-), becomes deficient for exonuclease activity and proficient as a recombinagenic helicase. Thus, the RecD Ejection model proposes that χ participates in recombination by acting as a toggle to convert RecBCD (a powerful exonuclease) to RecBC(D-) (a recombinase). We review the properties of RecBCD and its cognate site χ, including recent results that support both the RecD Ejection model and the view that χ plays only an indirect role in recombination.

Original languageEnglish (US)
Pages (from-to)49-70
Number of pages22
JournalAnnual Review of Genetics
Volume28
DOIs
StatePublished - Dec 1 1994
Externally publishedYes

Fingerprint

4 alpha-glucanotransferase
Exodeoxyribonuclease V
Exonucleases
Genetic Recombination
Escherichia coli
Recombinational DNA Repair
DNA
Recombinases
Genes
Proteins
Enzymes

Keywords

  • double chain breaks
  • exonuclease V
  • helicase
  • recD
  • recombination

ASJC Scopus subject areas

  • Genetics

Cite this

χ and the RecBC D enzyme of Escherichia coli. / Myers, Richard; Stahl, Franklin W.

In: Annual Review of Genetics, Vol. 28, 01.12.1994, p. 49-70.

Research output: Contribution to journalReview article

Myers, Richard ; Stahl, Franklin W. / χ and the RecBC D enzyme of Escherichia coli. In: Annual Review of Genetics. 1994 ; Vol. 28. pp. 49-70.
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AB - The products of genes recB and recC are responsible for conjugal recombination and for the repair of chromosomal double chain breaks in Escherichia coli. The product of the recD gene, which combines with the RecB and RecC proteins to comprise the RecBCD enzyme, is not required for either recombination or repair. On the contrary, RecBCD enzyme is a potent exonuclease which inhibits recombination by destroying linear DNA. The RecD Ejection model supposes that RecBCD enzyme enters DNA at a double-chain end and travels destructively along the DNA until (typically) it encounters the recombination hotspot sequence χ. χ then alters the RecBCD enzyme by weakening the affinity of the RecD subunit for the RecBC heterodimer. With the loss of the RecD subunit from the enzyme, the resulting protein, RecBC(D-), becomes deficient for exonuclease activity and proficient as a recombinagenic helicase. Thus, the RecD Ejection model proposes that χ participates in recombination by acting as a toggle to convert RecBCD (a powerful exonuclease) to RecBC(D-) (a recombinase). We review the properties of RecBCD and its cognate site χ, including recent results that support both the RecD Ejection model and the view that χ plays only an indirect role in recombination.

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