κ-Opiate binding to rat brain and guinea pig cerebellum: Sensitivity towards ions and nucleotides

Yossef Itzhak, Gavril W. Pasternak

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Sodium ions and guanine nucleotides markedly affect the binding to μ and δ sites. Using the guinea pig cerebellum, a tissue whose opioid binding sites are almost exclusively κ, we have examined the sensitivity of [3H](-)-ethylketocyclazocine ([3H]EKC) binding to ions and guanine nucleotides. Unlike μ and δ binding, sodium ions and guanine nucleotides had little effect on the [3H]EKC binding in the guinea pig cerebellum. On the other hand, calcium and manganese at 1 mM lowered [3H]EKC binding in the guinea pig cerebellum by approximately 25%. Binding in rat brain, which was primarily μ and δ, was unaffected. These results point out additional biochemical differences between κ sites and the classic μ and δ binding sites.

Original languageEnglish (US)
Pages (from-to)81-84
Number of pages4
JournalNeuroscience Letters
Volume64
Issue number1
DOIs
StatePublished - Feb 14 1986

Keywords

  • ethylketocyclazocine
  • guinea pig
  • morphine
  • opiate receptor
  • κ site

ASJC Scopus subject areas

  • Neuroscience(all)

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