Abstract
A new procedure for the measurement of γ-aminobutyric acid, norepinephrine, dopamine, serotonin and 5-hydroxyindoleacetic acid from the same brain region was developed. In general, two separate high-performance liquid chromatographic runs were performed, one for the γ-aminobutyric acid determination and one for the determination of the monoamines. The electrochemical detection of γ-aminobutyric acid was determined by a new procedure that utilized a small aliquot of the brain sample prepared for monoamine measurement. This assay was linear and parallel between 6 and 200 ng per 20-μl injection with 5-aminovaleric acid utilized as an internal standard. Inter-assay variability averaged 5% throughout the assay with γ-aminobutyric acid values in the gerbil hypothalamus of 344 μg/g. The catecholamine assay has been characterized previously and utilizes 3,4-dihydroxybenzylamine as an internal standard with less than 5% variability. Norepinephrine, dopamine, serotonin and 5-hydroxyindoleacetic acid levels in the gerbil hypothalamus averaged 2922, 729, 797 and 272 ng/g, respectively. This new protocol allows a wide range of neurochemicals to be determined and evaluated from the same brain region.
| Original language | English |
|---|---|
| Pages (from-to) | 334-339 |
| Number of pages | 6 |
| Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
| Volume | 579 |
| Issue number | 2 |
| DOIs | |
| State | Published - Sep 4 1992 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Chemistry(all)
- Clinical Biochemistry
- Molecular Medicine
Cite this
γ-Aminobutyric acid, catecholamine and indoleamine determinations from the same brain region by high-performance liquid chromatography with electrochemical detection. / Champney, Thomas; Hanneman, William H.; Nichols, Myesha A.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 579, No. 2, 04.09.1992, p. 334-339.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - γ-Aminobutyric acid, catecholamine and indoleamine determinations from the same brain region by high-performance liquid chromatography with electrochemical detection
AU - Champney, Thomas
AU - Hanneman, William H.
AU - Nichols, Myesha A.
PY - 1992/9/4
Y1 - 1992/9/4
N2 - A new procedure for the measurement of γ-aminobutyric acid, norepinephrine, dopamine, serotonin and 5-hydroxyindoleacetic acid from the same brain region was developed. In general, two separate high-performance liquid chromatographic runs were performed, one for the γ-aminobutyric acid determination and one for the determination of the monoamines. The electrochemical detection of γ-aminobutyric acid was determined by a new procedure that utilized a small aliquot of the brain sample prepared for monoamine measurement. This assay was linear and parallel between 6 and 200 ng per 20-μl injection with 5-aminovaleric acid utilized as an internal standard. Inter-assay variability averaged 5% throughout the assay with γ-aminobutyric acid values in the gerbil hypothalamus of 344 μg/g. The catecholamine assay has been characterized previously and utilizes 3,4-dihydroxybenzylamine as an internal standard with less than 5% variability. Norepinephrine, dopamine, serotonin and 5-hydroxyindoleacetic acid levels in the gerbil hypothalamus averaged 2922, 729, 797 and 272 ng/g, respectively. This new protocol allows a wide range of neurochemicals to be determined and evaluated from the same brain region.
AB - A new procedure for the measurement of γ-aminobutyric acid, norepinephrine, dopamine, serotonin and 5-hydroxyindoleacetic acid from the same brain region was developed. In general, two separate high-performance liquid chromatographic runs were performed, one for the γ-aminobutyric acid determination and one for the determination of the monoamines. The electrochemical detection of γ-aminobutyric acid was determined by a new procedure that utilized a small aliquot of the brain sample prepared for monoamine measurement. This assay was linear and parallel between 6 and 200 ng per 20-μl injection with 5-aminovaleric acid utilized as an internal standard. Inter-assay variability averaged 5% throughout the assay with γ-aminobutyric acid values in the gerbil hypothalamus of 344 μg/g. The catecholamine assay has been characterized previously and utilizes 3,4-dihydroxybenzylamine as an internal standard with less than 5% variability. Norepinephrine, dopamine, serotonin and 5-hydroxyindoleacetic acid levels in the gerbil hypothalamus averaged 2922, 729, 797 and 272 ng/g, respectively. This new protocol allows a wide range of neurochemicals to be determined and evaluated from the same brain region.
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UR - http://www.scopus.com/inward/citedby.url?scp=0026674474&partnerID=8YFLogxK
U2 - 10.1016/0378-4347(92)80400-K
DO - 10.1016/0378-4347(92)80400-K
M3 - Article
VL - 579
SP - 334
EP - 339
JO - Journal of Chromatography - Biomedical Applications
JF - Journal of Chromatography - Biomedical Applications
SN - 0378-4347
IS - 2
ER -