β1 Adrenergic receptor is key to cold-and diet-induced thermogenesis in mice

Cintia B. Ueta, Gustavo W. Fernandes, Luciane P. Capelo, Tatiane L. Fonseca, Flávia D Angelo Maculan, Cecilia H A Gouveia, Patrícia C. Brum, Marcelo A. Christoffolete, Marcelo S. Aoki, Carmen L. Lancellotti, Brian Kim, Antonio C. Bianco, Miriam O. Ribeiro

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Brown adipose tissue (BAT) is predominantly regulated by the sympathetic nervous system (SNS) and the adrenergic receptor signaling pathway. Knowing that a mouse with triple β-receptor knockout (KO) is cold intolerant and obese, we evaluated the independent role played by the β1 isoform in energy homeostasis. First, the 30 min i.v. infusion of norepinephrine (NE) or the β1 selective agonist dobutamine (DB) resulted in similar interscapular BAT (iBAT) thermal response in WT mice. Secondly, mice with targeted disruption of the β1 gene (KO of β1 adrenergic receptor (β1KO)) developed hypothermia during cold exposure and exhibited decreased iBAT thermal response to NE or DB infusion. Thirdly, when placed on a high-fat diet (HFD; 40% fat) for 5 weeks, β1KO mice were more susceptible to obesity than WT controls and failed to develop diet-induced thermogenesis as assessed by BAT Ucp1 mRNA levels and oxygen consumption. Furthermore, β1KO mice exhibited fasting hyperglycemia and more intense glucose intolerance, hypercholesterolemia, and hypertriglyceridemia when placed on the HFD, developing marked non-alcoholic steatohepatitis. In conclusion, the β1 signaling pathway mediates most of the SNS stimulation of adaptive thermogenesis.

Original languageEnglish
Pages (from-to)359-365
Number of pages7
JournalJournal of Endocrinology
Volume214
Issue number3
DOIs
StatePublished - Sep 1 2012

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Thermogenesis
Adrenergic Receptors
Brown Adipose Tissue
Diet
Dobutamine
Sympathetic Nervous System
Norepinephrine
Hot Temperature
Gene Knockout Techniques
Glucose Intolerance
Hypertriglyceridemia
High Fat Diet
Fatty Liver
Hypercholesterolemia
Hypothermia
Oxygen Consumption
Hyperglycemia
Fasting
Protein Isoforms
Homeostasis

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Ueta, C. B., Fernandes, G. W., Capelo, L. P., Fonseca, T. L., Maculan, F. D. A., Gouveia, C. H. A., ... Ribeiro, M. O. (2012). β1 Adrenergic receptor is key to cold-and diet-induced thermogenesis in mice. Journal of Endocrinology, 214(3), 359-365. https://doi.org/10.1530/JOE-12-0155

β1 Adrenergic receptor is key to cold-and diet-induced thermogenesis in mice. / Ueta, Cintia B.; Fernandes, Gustavo W.; Capelo, Luciane P.; Fonseca, Tatiane L.; Maculan, Flávia D Angelo; Gouveia, Cecilia H A; Brum, Patrícia C.; Christoffolete, Marcelo A.; Aoki, Marcelo S.; Lancellotti, Carmen L.; Kim, Brian; Bianco, Antonio C.; Ribeiro, Miriam O.

In: Journal of Endocrinology, Vol. 214, No. 3, 01.09.2012, p. 359-365.

Research output: Contribution to journalArticle

Ueta, CB, Fernandes, GW, Capelo, LP, Fonseca, TL, Maculan, FDA, Gouveia, CHA, Brum, PC, Christoffolete, MA, Aoki, MS, Lancellotti, CL, Kim, B, Bianco, AC & Ribeiro, MO 2012, 'β1 Adrenergic receptor is key to cold-and diet-induced thermogenesis in mice', Journal of Endocrinology, vol. 214, no. 3, pp. 359-365. https://doi.org/10.1530/JOE-12-0155
Ueta CB, Fernandes GW, Capelo LP, Fonseca TL, Maculan FDA, Gouveia CHA et al. β1 Adrenergic receptor is key to cold-and diet-induced thermogenesis in mice. Journal of Endocrinology. 2012 Sep 1;214(3):359-365. https://doi.org/10.1530/JOE-12-0155
Ueta, Cintia B. ; Fernandes, Gustavo W. ; Capelo, Luciane P. ; Fonseca, Tatiane L. ; Maculan, Flávia D Angelo ; Gouveia, Cecilia H A ; Brum, Patrícia C. ; Christoffolete, Marcelo A. ; Aoki, Marcelo S. ; Lancellotti, Carmen L. ; Kim, Brian ; Bianco, Antonio C. ; Ribeiro, Miriam O. / β1 Adrenergic receptor is key to cold-and diet-induced thermogenesis in mice. In: Journal of Endocrinology. 2012 ; Vol. 214, No. 3. pp. 359-365.
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