β-Glucosylarginine: a new glucose-protein bond in a self-glucosylating protein from sweet corn

David G. Singh, Joseph Lomako, Wieslawa M. Lomako, William J. Whelan, Helmut E. Meyer, Maria Serwe, Jörg W. Metzger

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

In the search for a protein primer for starch synthesis, an autocatalytic self-glucosylating protein has been isolated from sweet corn. Several tryptic peptides were obtained from the [14C]glucosylated protein and were sequenced, corresponding to over 40% of the estimated total sequence (molecular mass 42 kDa). There is no homology with the amino acid sequence of the autocatalytic glycogen primer, glycogenin, nor in respect of the nature of the union between the autocatalytically added glucose and the protein, which, in the case of the corn protein, now named amylogenin, is a novel glucose-protein bond, a single β-glucose residue joined to an arginine residue.

Original languageEnglish
Pages (from-to)61-64
Number of pages4
JournalFEBS Letters
Volume376
Issue number1-2
DOIs
StatePublished - Nov 27 1995

Fingerprint

Zea mays
Glucose
Proteins
Amino Acid Sequence Homology
Molecular mass
Glycogen
Starch
Arginine
glucosylarginine
Amino Acids
Peptides

Keywords

  • Amylogenin
  • Autoglucosylation
  • Self-glucosylating protein
  • β-Glucosylarginine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Singh, D. G., Lomako, J., Lomako, W. M., Whelan, W. J., Meyer, H. E., Serwe, M., & Metzger, J. W. (1995). β-Glucosylarginine: a new glucose-protein bond in a self-glucosylating protein from sweet corn. FEBS Letters, 376(1-2), 61-64. https://doi.org/10.1016/0014-5793(95)01247-6

β-Glucosylarginine : a new glucose-protein bond in a self-glucosylating protein from sweet corn. / Singh, David G.; Lomako, Joseph; Lomako, Wieslawa M.; Whelan, William J.; Meyer, Helmut E.; Serwe, Maria; Metzger, Jörg W.

In: FEBS Letters, Vol. 376, No. 1-2, 27.11.1995, p. 61-64.

Research output: Contribution to journalArticle

Singh, DG, Lomako, J, Lomako, WM, Whelan, WJ, Meyer, HE, Serwe, M & Metzger, JW 1995, 'β-Glucosylarginine: a new glucose-protein bond in a self-glucosylating protein from sweet corn', FEBS Letters, vol. 376, no. 1-2, pp. 61-64. https://doi.org/10.1016/0014-5793(95)01247-6
Singh, David G. ; Lomako, Joseph ; Lomako, Wieslawa M. ; Whelan, William J. ; Meyer, Helmut E. ; Serwe, Maria ; Metzger, Jörg W. / β-Glucosylarginine : a new glucose-protein bond in a self-glucosylating protein from sweet corn. In: FEBS Letters. 1995 ; Vol. 376, No. 1-2. pp. 61-64.
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