Abstract
The Escherichia coli gene gusA was expressed in the methylotrophic yeast Pichia pastoris in a transcriptional fusion to the homologous methanol-inducible AOX1 promoter. Four recombinant clones were selected for expression studies in shake flask conditions and β-D-glucuronidase (β-GUS) activity was assayed each 24 h during the induction period. Regardless of the genomic integration patterns and the gene dosage, β-GUS was functionally expressed and easily detected in all studied clones. The results obtained demonstrate the feasibility of using this bacterial enzyme as a reporter in Pichia pastoris.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 278-283 |
| Number of pages | 6 |
| Journal | Letters in Applied Microbiology |
| Volume | 29 |
| Issue number | 5 |
| DOIs | |
| State | Published - Dec 1 1999 |
| Externally published | Yes |
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
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Cite this
Ayra-Pardo, C., Montejo-Sierra, I. L., Väzquez-Padrön, R. I., & García-Martínez, C. (1999). β-D-glucuronidase gene from Escherichia coli is a functional reporter in the methylotrophic yeast Pichia pastoris. Letters in Applied Microbiology, 29(5), 278-283. https://doi.org/10.1046/j.1365-2672.1999.00610.x