β-D-glucuronidase gene from Escherichia coli is a functional reporter in the methylotrophic yeast Pichia pastoris

C. Ayra-Pardo, I. L. Montejo-Sierra, R. I. Väzquez-Padrön, C. García-Martínez

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The Escherichia coli gene gusA was expressed in the methylotrophic yeast Pichia pastoris in a transcriptional fusion to the homologous methanol-inducible AOX1 promoter. Four recombinant clones were selected for expression studies in shake flask conditions and β-D-glucuronidase (β-GUS) activity was assayed each 24 h during the induction period. Regardless of the genomic integration patterns and the gene dosage, β-GUS was functionally expressed and easily detected in all studied clones. The results obtained demonstrate the feasibility of using this bacterial enzyme as a reporter in Pichia pastoris.

Original languageEnglish (US)
Pages (from-to)278-283
Number of pages6
JournalLetters in Applied Microbiology
Volume29
Issue number5
DOIs
StatePublished - Dec 1 1999
Externally publishedYes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'β-D-glucuronidase gene from Escherichia coli is a functional reporter in the methylotrophic yeast Pichia pastoris'. Together they form a unique fingerprint.

  • Cite this