β-D-glucuronidase gene from Escherichia coli is a functional reporter in the methylotrophic yeast Pichia pastoris

C. Ayra-Pardo; I. L. Montejo-Sierra; R. I. Väzquez-Padrön; C. García-Martínez

doi:10.1046/j.1365-2672.1999.00610.x

β-D-glucuronidase gene from Escherichia coli is a functional reporter in the methylotrophic yeast Pichia pastoris

C. Ayra-Pardo, I. L. Montejo-Sierra, R. I. Väzquez-Padrön, C. García-Martínez

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The Escherichia coli gene gusA was expressed in the methylotrophic yeast Pichia pastoris in a transcriptional fusion to the homologous methanol-inducible AOX1 promoter. Four recombinant clones were selected for expression studies in shake flask conditions and β-D-glucuronidase (β-GUS) activity was assayed each 24 h during the induction period. Regardless of the genomic integration patterns and the gene dosage, β-GUS was functionally expressed and easily detected in all studied clones. The results obtained demonstrate the feasibility of using this bacterial enzyme as a reporter in Pichia pastoris.

Original language	English (US)
Pages (from-to)	278-283
Number of pages	6
Journal	Letters in Applied Microbiology
Volume	29
Issue number	5
DOIs	https://doi.org/10.1046/j.1365-2672.1999.00610.x
State	Published - 1999
Externally published	Yes

ASJC Scopus subject areas

Applied Microbiology and Biotechnology

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title = "β-D-glucuronidase gene from Escherichia coli is a functional reporter in the methylotrophic yeast Pichia pastoris",

abstract = "The Escherichia coli gene gusA was expressed in the methylotrophic yeast Pichia pastoris in a transcriptional fusion to the homologous methanol-inducible AOX1 promoter. Four recombinant clones were selected for expression studies in shake flask conditions and β-D-glucuronidase (β-GUS) activity was assayed each 24 h during the induction period. Regardless of the genomic integration patterns and the gene dosage, β-GUS was functionally expressed and easily detected in all studied clones. The results obtained demonstrate the feasibility of using this bacterial enzyme as a reporter in Pichia pastoris.",

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AU - Väzquez-Padrön, R. I.

AU - García-Martínez, C.

PY - 1999

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AB - The Escherichia coli gene gusA was expressed in the methylotrophic yeast Pichia pastoris in a transcriptional fusion to the homologous methanol-inducible AOX1 promoter. Four recombinant clones were selected for expression studies in shake flask conditions and β-D-glucuronidase (β-GUS) activity was assayed each 24 h during the induction period. Regardless of the genomic integration patterns and the gene dosage, β-GUS was functionally expressed and easily detected in all studied clones. The results obtained demonstrate the feasibility of using this bacterial enzyme as a reporter in Pichia pastoris.

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