Abstract
The Escherichia coli gene gusA was expressed in the methylotrophic yeast Pichia pastoris in a transcriptional fusion to the homologous methanol-inducible AOX1 promoter. Four recombinant clones were selected for expression studies in shake flask conditions and β-D-glucuronidase (β-GUS) activity was assayed each 24 h during the induction period. Regardless of the genomic integration patterns and the gene dosage, β-GUS was functionally expressed and easily detected in all studied clones. The results obtained demonstrate the feasibility of using this bacterial enzyme as a reporter in Pichia pastoris.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 278-283 |
| Number of pages | 6 |
| Journal | Letters in Applied Microbiology |
| Volume | 29 |
| Issue number | 5 |
| DOIs | |
| State | Published - 1999 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Water Science and Technology
- Fluid Flow and Transfer Processes
- Microbiology
- Ecology
Cite this
β-D-glucuronidase gene from Escherichia coli is a functional reporter in the methylotrophic yeast Pichia pastoris. / Ayra-Pardo, C.; Montejo-Sierra, I. L.; Vazquez-Padron, Roberto I; García-Martínez, C.
In: Letters in Applied Microbiology, Vol. 29, No. 5, 1999, p. 278-283.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - β-D-glucuronidase gene from Escherichia coli is a functional reporter in the methylotrophic yeast Pichia pastoris
AU - Ayra-Pardo, C.
AU - Montejo-Sierra, I. L.
AU - Vazquez-Padron, Roberto I
AU - García-Martínez, C.
PY - 1999
Y1 - 1999
N2 - The Escherichia coli gene gusA was expressed in the methylotrophic yeast Pichia pastoris in a transcriptional fusion to the homologous methanol-inducible AOX1 promoter. Four recombinant clones were selected for expression studies in shake flask conditions and β-D-glucuronidase (β-GUS) activity was assayed each 24 h during the induction period. Regardless of the genomic integration patterns and the gene dosage, β-GUS was functionally expressed and easily detected in all studied clones. The results obtained demonstrate the feasibility of using this bacterial enzyme as a reporter in Pichia pastoris.
AB - The Escherichia coli gene gusA was expressed in the methylotrophic yeast Pichia pastoris in a transcriptional fusion to the homologous methanol-inducible AOX1 promoter. Four recombinant clones were selected for expression studies in shake flask conditions and β-D-glucuronidase (β-GUS) activity was assayed each 24 h during the induction period. Regardless of the genomic integration patterns and the gene dosage, β-GUS was functionally expressed and easily detected in all studied clones. The results obtained demonstrate the feasibility of using this bacterial enzyme as a reporter in Pichia pastoris.
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UR - http://www.scopus.com/inward/citedby.url?scp=0033426876&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2672.1999.00610.x
DO - 10.1046/j.1365-2672.1999.00610.x
M3 - Article
VL - 29
SP - 278
EP - 283
JO - Letters in Applied Microbiology
JF - Letters in Applied Microbiology
SN - 0266-8254
IS - 5
ER -