β-D-glucuronidase gene from Escherichia coli is a functional reporter in the methylotrophic yeast Pichia pastoris

C. Ayra-Pardo, I. L. Montejo-Sierra, Roberto I Vazquez-Padron, C. García-Martínez

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The Escherichia coli gene gusA was expressed in the methylotrophic yeast Pichia pastoris in a transcriptional fusion to the homologous methanol-inducible AOX1 promoter. Four recombinant clones were selected for expression studies in shake flask conditions and β-D-glucuronidase (β-GUS) activity was assayed each 24 h during the induction period. Regardless of the genomic integration patterns and the gene dosage, β-GUS was functionally expressed and easily detected in all studied clones. The results obtained demonstrate the feasibility of using this bacterial enzyme as a reporter in Pichia pastoris.

Original languageEnglish (US)
Pages (from-to)278-283
Number of pages6
JournalLetters in Applied Microbiology
Volume29
Issue number5
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Pichia
Glucuronidase
Yeast
Escherichia coli
yeast
clone
Clone Cells
Genes
Yeasts
Gene Dosage
gene
Methanol
methanol
genomics
Fusion reactions
Enzymes
enzyme
dosage

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Water Science and Technology
  • Fluid Flow and Transfer Processes
  • Microbiology
  • Ecology

Cite this

β-D-glucuronidase gene from Escherichia coli is a functional reporter in the methylotrophic yeast Pichia pastoris. / Ayra-Pardo, C.; Montejo-Sierra, I. L.; Vazquez-Padron, Roberto I; García-Martínez, C.

In: Letters in Applied Microbiology, Vol. 29, No. 5, 1999, p. 278-283.

Research output: Contribution to journalArticle

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