TY - JOUR
T1 - β-D-glucuronidase gene from Escherichia coli is a functional reporter in the methylotrophic yeast Pichia pastoris
AU - Ayra-Pardo, C.
AU - Montejo-Sierra, I. L.
AU - Väzquez-Padrön, R. I.
AU - García-Martínez, C.
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1999
Y1 - 1999
N2 - The Escherichia coli gene gusA was expressed in the methylotrophic yeast Pichia pastoris in a transcriptional fusion to the homologous methanol-inducible AOX1 promoter. Four recombinant clones were selected for expression studies in shake flask conditions and β-D-glucuronidase (β-GUS) activity was assayed each 24 h during the induction period. Regardless of the genomic integration patterns and the gene dosage, β-GUS was functionally expressed and easily detected in all studied clones. The results obtained demonstrate the feasibility of using this bacterial enzyme as a reporter in Pichia pastoris.
AB - The Escherichia coli gene gusA was expressed in the methylotrophic yeast Pichia pastoris in a transcriptional fusion to the homologous methanol-inducible AOX1 promoter. Four recombinant clones were selected for expression studies in shake flask conditions and β-D-glucuronidase (β-GUS) activity was assayed each 24 h during the induction period. Regardless of the genomic integration patterns and the gene dosage, β-GUS was functionally expressed and easily detected in all studied clones. The results obtained demonstrate the feasibility of using this bacterial enzyme as a reporter in Pichia pastoris.
UR - http://www.scopus.com/inward/record.url?scp=0033426876&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033426876&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2672.1999.00610.x
DO - 10.1046/j.1365-2672.1999.00610.x
M3 - Article
C2 - 10664965
AN - SCOPUS:0033426876
VL - 29
SP - 278
EP - 283
JO - Letters in Applied Microbiology
JF - Letters in Applied Microbiology
SN - 0266-8254
IS - 5
ER -