β-catenin expression is altered in human colonic aberrant crypt foci

Pei Hao Xing Pei Hao, T. G. Pretlow, Jonnagadda S Rao, T. P. Pretlow

Research output: Contribution to journalArticle

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Abstract

The aberrant expression of β-catenin in colon tumors and the discovery of β-catenin mutations in small adenomas suggest that alterations of β-catenin are early events in human colorectal carcinogenesis. Here, we describe the expression of β-catenin in human aberrant crypt foci (ACF), the earliest identified neoplastic lesions in the colon. Paraffin-embedded sections of 94 ACF, 12 adenomas, and 10 carcinomas were evaluated for β-catenin expression by immunohistochemistry. Normal colonic epithelial cells adjacent to these lesions showed strong membranous expression of β-catenin and lacked cytoplasmic and nuclear expression. Cytoplasmic expression of β-catenin was seen in 25 of 46 ACF with dysplasia and in 2 of 48 ACF with atypia. In ACF with dysplasia, reduced membranous expression of β-catenin was associated with increased nuclear (P = 0.0013) and cytoplasmic (P = 0.0247) expression. The membranous (P = 0.0003) expression of β-catenin was reduced, and the cytoplasmic (P = 0.0016) and nuclear (P = 0.0266) expressions increased in ACF according to their degree of dysplasia. Likewise, membranous (P = 0.0007) expression of β-catenin was reduced, and the cytoplasmic (P = 0.0050) and nuclear (P = 0.0001) expressions increased from ACF to adenoma to carcinoma. These data suggest that ACF and their aberrant expression of β-catenin play a role in colon tumorigenesis.

Original languageEnglish
Pages (from-to)8085-8088
Number of pages4
JournalCancer Research
Volume61
Issue number22
StatePublished - Nov 15 2001
Externally publishedYes

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Aberrant Crypt Foci
Catenins
Adenoma
Colon
Carcinogenesis
Carcinoma
Paraffin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Xing Pei Hao, P. H., Pretlow, T. G., Rao, J. S., & Pretlow, T. P. (2001). β-catenin expression is altered in human colonic aberrant crypt foci. Cancer Research, 61(22), 8085-8088.

β-catenin expression is altered in human colonic aberrant crypt foci. / Xing Pei Hao, Pei Hao; Pretlow, T. G.; Rao, Jonnagadda S; Pretlow, T. P.

In: Cancer Research, Vol. 61, No. 22, 15.11.2001, p. 8085-8088.

Research output: Contribution to journalArticle

Xing Pei Hao, PH, Pretlow, TG, Rao, JS & Pretlow, TP 2001, 'β-catenin expression is altered in human colonic aberrant crypt foci', Cancer Research, vol. 61, no. 22, pp. 8085-8088.
Xing Pei Hao PH, Pretlow TG, Rao JS, Pretlow TP. β-catenin expression is altered in human colonic aberrant crypt foci. Cancer Research. 2001 Nov 15;61(22):8085-8088.
Xing Pei Hao, Pei Hao ; Pretlow, T. G. ; Rao, Jonnagadda S ; Pretlow, T. P. / β-catenin expression is altered in human colonic aberrant crypt foci. In: Cancer Research. 2001 ; Vol. 61, No. 22. pp. 8085-8088.
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