Abstract
The aberrant expression of β-catenin in colon tumors and the discovery of β-catenin mutations in small adenomas suggest that alterations of β-catenin are early events in human colorectal carcinogenesis. Here, we describe the expression of β-catenin in human aberrant crypt foci (ACF), the earliest identified neoplastic lesions in the colon. Paraffin-embedded sections of 94 ACF, 12 adenomas, and 10 carcinomas were evaluated for β-catenin expression by immunohistochemistry. Normal colonic epithelial cells adjacent to these lesions showed strong membranous expression of β-catenin and lacked cytoplasmic and nuclear expression. Cytoplasmic expression of β-catenin was seen in 25 of 46 ACF with dysplasia and in 2 of 48 ACF with atypia. In ACF with dysplasia, reduced membranous expression of β-catenin was associated with increased nuclear (P = 0.0013) and cytoplasmic (P = 0.0247) expression. The membranous (P = 0.0003) expression of β-catenin was reduced, and the cytoplasmic (P = 0.0016) and nuclear (P = 0.0266) expressions increased in ACF according to their degree of dysplasia. Likewise, membranous (P = 0.0007) expression of β-catenin was reduced, and the cytoplasmic (P = 0.0050) and nuclear (P = 0.0001) expressions increased from ACF to adenoma to carcinoma. These data suggest that ACF and their aberrant expression of β-catenin play a role in colon tumorigenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 8085-8088 |
| Number of pages | 4 |
| Journal | Cancer Research |
| Volume | 61 |
| Issue number | 22 |
| State | Published - Nov 15 2001 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
β-catenin expression is altered in human colonic aberrant crypt foci. / Xing Pei Hao, Pei Hao; Pretlow, T. G.; Rao, Jonnagadda S; Pretlow, T. P.
In: Cancer Research, Vol. 61, No. 22, 15.11.2001, p. 8085-8088.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - β-catenin expression is altered in human colonic aberrant crypt foci
AU - Xing Pei Hao, Pei Hao
AU - Pretlow, T. G.
AU - Rao, Jonnagadda S
AU - Pretlow, T. P.
PY - 2001/11/15
Y1 - 2001/11/15
N2 - The aberrant expression of β-catenin in colon tumors and the discovery of β-catenin mutations in small adenomas suggest that alterations of β-catenin are early events in human colorectal carcinogenesis. Here, we describe the expression of β-catenin in human aberrant crypt foci (ACF), the earliest identified neoplastic lesions in the colon. Paraffin-embedded sections of 94 ACF, 12 adenomas, and 10 carcinomas were evaluated for β-catenin expression by immunohistochemistry. Normal colonic epithelial cells adjacent to these lesions showed strong membranous expression of β-catenin and lacked cytoplasmic and nuclear expression. Cytoplasmic expression of β-catenin was seen in 25 of 46 ACF with dysplasia and in 2 of 48 ACF with atypia. In ACF with dysplasia, reduced membranous expression of β-catenin was associated with increased nuclear (P = 0.0013) and cytoplasmic (P = 0.0247) expression. The membranous (P = 0.0003) expression of β-catenin was reduced, and the cytoplasmic (P = 0.0016) and nuclear (P = 0.0266) expressions increased in ACF according to their degree of dysplasia. Likewise, membranous (P = 0.0007) expression of β-catenin was reduced, and the cytoplasmic (P = 0.0050) and nuclear (P = 0.0001) expressions increased from ACF to adenoma to carcinoma. These data suggest that ACF and their aberrant expression of β-catenin play a role in colon tumorigenesis.
AB - The aberrant expression of β-catenin in colon tumors and the discovery of β-catenin mutations in small adenomas suggest that alterations of β-catenin are early events in human colorectal carcinogenesis. Here, we describe the expression of β-catenin in human aberrant crypt foci (ACF), the earliest identified neoplastic lesions in the colon. Paraffin-embedded sections of 94 ACF, 12 adenomas, and 10 carcinomas were evaluated for β-catenin expression by immunohistochemistry. Normal colonic epithelial cells adjacent to these lesions showed strong membranous expression of β-catenin and lacked cytoplasmic and nuclear expression. Cytoplasmic expression of β-catenin was seen in 25 of 46 ACF with dysplasia and in 2 of 48 ACF with atypia. In ACF with dysplasia, reduced membranous expression of β-catenin was associated with increased nuclear (P = 0.0013) and cytoplasmic (P = 0.0247) expression. The membranous (P = 0.0003) expression of β-catenin was reduced, and the cytoplasmic (P = 0.0016) and nuclear (P = 0.0266) expressions increased in ACF according to their degree of dysplasia. Likewise, membranous (P = 0.0007) expression of β-catenin was reduced, and the cytoplasmic (P = 0.0050) and nuclear (P = 0.0001) expressions increased from ACF to adenoma to carcinoma. These data suggest that ACF and their aberrant expression of β-catenin play a role in colon tumorigenesis.
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M3 - Article
VL - 61
SP - 8085
EP - 8088
JO - Journal of Cancer Research
JF - Journal of Cancer Research
SN - 0099-7013
IS - 22
ER -