α-tocopheryl phosphate: A novel, natural form of vitamin E

Robert Gianello, Roksan Libinaki, Angelo Azzi, Paul D. Gavin, Yesim Negis, Jean Marc Zingg, Phillip Holt, Hooi Hong Keah, Annike Griffey, Andrew Smallridge, Simon M. West, Esra Ogru

Research output: Contribution to journalArticlepeer-review

74 Scopus citations

Abstract

We have detected α-tocopheryl phosphate in biological tissues including liver and adipose tissue, as well as in a variety of foods, suggesting a ubiquitous presence in animal and plant tissue. α-Tocopheryl phosphate is a water-soluble molecule that is resistant to both acid and alkaline hydrolysis, making it undetectable using standard assays for vitamin E. A new method was therefore developed to allow the extraction of both α-tocopheryl phosphate and α-tocopherol from a single specimen. We used ESMS to detect endogenous α-tocopheryl phosphate in biological samples that also contained α-tocopherol. Due to the significance of these findings, further proof was required to unequivocally demonstrate the presence of endogenous α-tocopheryl phosphate in biological samples. Four independent methods of analysis were examined: HPLC, LCMS, LCMS/MS, and GCMS. α-Tocopherol phosphate was identified in all instances by comparison between standard α-tocopheryl phosphate and extracts of biological tissues. The results show that α-tocopheryl phosphate is a natural form of vitamin E. The discovery of endogenous α-tocopheryl phosphate has implications for the expanding knowledge of the roles of α-tocopherol in biological systems.

Original languageEnglish (US)
Pages (from-to)970-976
Number of pages7
JournalFree Radical Biology and Medicine
Volume39
Issue number7
DOIs
StatePublished - Oct 1 2005
Externally publishedYes

Keywords

  • Biological tissues
  • Free radicals
  • Vitamin E
  • Vitamin E phosphate
  • α-Tocopheryl phosphate

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

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