α-Tocopherol dietary supplement decreases titers of antibody against 5- hydroxymethyl-2'-deoxyuridine (HMdU)

Jennifer Hu, Chuan Xiang Chi, Krystyna Frenkel, Brian N. Smith, John J. Henfelt, Marianne Berwick, Somdat Mahabir, Ralph B. D'Agostino

Research output: Contribution to journalArticle

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Abstract

This study evaluated the effects of vitamin E (α-tocopherol) on oxidative DNA damage in a randomized double-blind Phase II chemoprevention trial. Oxidative DNA damage was measured by the level of auto-antibody (Ab) against 5-hydroxymethyl-2'-deoxyuridine (HMdU) in plasma. After the baseline screening, eligible subjects (n = 31; plasma samples from 28 subjects were available for this study) were randomized to receive 15, 60, or 200 mg of α- tocopherol per day for 28 days. Biomarkers were measured twice at baseline - on day 1 (visit 1) and day 3 (visit 2) - and twice after intervention - on day 17 (visit 3) and day 31 (visit 4). At baseline, there was a highly significant inverse correlation between anti-HMdU Ab titer and plasma vitamin E level (r = -0.53; P = 0.004; n = 28). Smoking did not affect baseline anti- HMdU Ab titer; however, anti-HMdU Ab titer levels at baseline were significantly lower in subjects with above-median (0.75 ounce/day) alcohol consumption (P = 0.008). No significant change in anti-HMdU Ab level occurred at either visit 3 or visit 4 for subjects on the lowest dose, 15 mg α- tocopherol per day. Subjects receiving 60 mg of α-tocopherol per day had a significant decrease in anti-HMdU Ab level at visits 3 and 4 compared with baseline (P = 0.049 and P = 0.02, respectively). However, subjects receiving the highest dose, 200 mg/day, had less consistent results: a significant decrease in anti-HMdU Ab level was seen at visit 4 (P = 0.04) but not at visit 3. Our results demonstrate an inverse relationship between α- tocopherol and anti-HMdU Abs in plasma; oxidative DNA damage can be modulated by short-term dietary supplementation of α-tocopherol in some subjects.

Original languageEnglish
Pages (from-to)693-698
Number of pages6
JournalCancer Epidemiology Biomarkers and Prevention
Volume8
Issue number8
StatePublished - Aug 1 1999
Externally publishedYes

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Deoxyuridine
Tocopherols
Dietary Supplements
Antibodies
DNA Damage
Vitamin E
Chemoprevention
5-hydroxymethyl-2'-deoxyuridine
Alcohol Drinking
Biomarkers
Smoking

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

Cite this

Hu, J., Chi, C. X., Frenkel, K., Smith, B. N., Henfelt, J. J., Berwick, M., ... D'Agostino, R. B. (1999). α-Tocopherol dietary supplement decreases titers of antibody against 5- hydroxymethyl-2'-deoxyuridine (HMdU). Cancer Epidemiology Biomarkers and Prevention, 8(8), 693-698.

α-Tocopherol dietary supplement decreases titers of antibody against 5- hydroxymethyl-2'-deoxyuridine (HMdU). / Hu, Jennifer; Chi, Chuan Xiang; Frenkel, Krystyna; Smith, Brian N.; Henfelt, John J.; Berwick, Marianne; Mahabir, Somdat; D'Agostino, Ralph B.

In: Cancer Epidemiology Biomarkers and Prevention, Vol. 8, No. 8, 01.08.1999, p. 693-698.

Research output: Contribution to journalArticle

Hu, J, Chi, CX, Frenkel, K, Smith, BN, Henfelt, JJ, Berwick, M, Mahabir, S & D'Agostino, RB 1999, 'α-Tocopherol dietary supplement decreases titers of antibody against 5- hydroxymethyl-2'-deoxyuridine (HMdU)', Cancer Epidemiology Biomarkers and Prevention, vol. 8, no. 8, pp. 693-698.
Hu, Jennifer ; Chi, Chuan Xiang ; Frenkel, Krystyna ; Smith, Brian N. ; Henfelt, John J. ; Berwick, Marianne ; Mahabir, Somdat ; D'Agostino, Ralph B. / α-Tocopherol dietary supplement decreases titers of antibody against 5- hydroxymethyl-2'-deoxyuridine (HMdU). In: Cancer Epidemiology Biomarkers and Prevention. 1999 ; Vol. 8, No. 8. pp. 693-698.
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abstract = "This study evaluated the effects of vitamin E (α-tocopherol) on oxidative DNA damage in a randomized double-blind Phase II chemoprevention trial. Oxidative DNA damage was measured by the level of auto-antibody (Ab) against 5-hydroxymethyl-2'-deoxyuridine (HMdU) in plasma. After the baseline screening, eligible subjects (n = 31; plasma samples from 28 subjects were available for this study) were randomized to receive 15, 60, or 200 mg of α- tocopherol per day for 28 days. Biomarkers were measured twice at baseline - on day 1 (visit 1) and day 3 (visit 2) - and twice after intervention - on day 17 (visit 3) and day 31 (visit 4). At baseline, there was a highly significant inverse correlation between anti-HMdU Ab titer and plasma vitamin E level (r = -0.53; P = 0.004; n = 28). Smoking did not affect baseline anti- HMdU Ab titer; however, anti-HMdU Ab titer levels at baseline were significantly lower in subjects with above-median (0.75 ounce/day) alcohol consumption (P = 0.008). No significant change in anti-HMdU Ab level occurred at either visit 3 or visit 4 for subjects on the lowest dose, 15 mg α- tocopherol per day. Subjects receiving 60 mg of α-tocopherol per day had a significant decrease in anti-HMdU Ab level at visits 3 and 4 compared with baseline (P = 0.049 and P = 0.02, respectively). However, subjects receiving the highest dose, 200 mg/day, had less consistent results: a significant decrease in anti-HMdU Ab level was seen at visit 4 (P = 0.04) but not at visit 3. Our results demonstrate an inverse relationship between α- tocopherol and anti-HMdU Abs in plasma; oxidative DNA damage can be modulated by short-term dietary supplementation of α-tocopherol in some subjects.",
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AU - Henfelt, John J.

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