α-Tocopherol dietary supplement decreases titers of antibody against 5- hydroxymethyl-2'-deoxyuridine (HMdU)

Jennifer J. Hu, Chuan Xiang Chi, Krystyna Frenkel, Brian N. Smith, John J. Henfelt, Marianne Berwick, Somdat Mahabir, Ralph B. D'Agostino

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

This study evaluated the effects of vitamin E (α-tocopherol) on oxidative DNA damage in a randomized double-blind Phase II chemoprevention trial. Oxidative DNA damage was measured by the level of auto-antibody (Ab) against 5-hydroxymethyl-2'-deoxyuridine (HMdU) in plasma. After the baseline screening, eligible subjects (n = 31; plasma samples from 28 subjects were available for this study) were randomized to receive 15, 60, or 200 mg of α- tocopherol per day for 28 days. Biomarkers were measured twice at baseline - on day 1 (visit 1) and day 3 (visit 2) - and twice after intervention - on day 17 (visit 3) and day 31 (visit 4). At baseline, there was a highly significant inverse correlation between anti-HMdU Ab titer and plasma vitamin E level (r = -0.53; P = 0.004; n = 28). Smoking did not affect baseline anti- HMdU Ab titer; however, anti-HMdU Ab titer levels at baseline were significantly lower in subjects with above-median (0.75 ounce/day) alcohol consumption (P = 0.008). No significant change in anti-HMdU Ab level occurred at either visit 3 or visit 4 for subjects on the lowest dose, 15 mg α- tocopherol per day. Subjects receiving 60 mg of α-tocopherol per day had a significant decrease in anti-HMdU Ab level at visits 3 and 4 compared with baseline (P = 0.049 and P = 0.02, respectively). However, subjects receiving the highest dose, 200 mg/day, had less consistent results: a significant decrease in anti-HMdU Ab level was seen at visit 4 (P = 0.04) but not at visit 3. Our results demonstrate an inverse relationship between α- tocopherol and anti-HMdU Abs in plasma; oxidative DNA damage can be modulated by short-term dietary supplementation of α-tocopherol in some subjects.

Original languageEnglish (US)
Pages (from-to)693-698
Number of pages6
JournalCancer Epidemiology Biomarkers and Prevention
Volume8
Issue number8
StatePublished - Aug 1999

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

Fingerprint Dive into the research topics of 'α-Tocopherol dietary supplement decreases titers of antibody against 5- hydroxymethyl-2'-deoxyuridine (HMdU)'. Together they form a unique fingerprint.

  • Cite this

    Hu, J. J., Chi, C. X., Frenkel, K., Smith, B. N., Henfelt, J. J., Berwick, M., Mahabir, S., & D'Agostino, R. B. (1999). α-Tocopherol dietary supplement decreases titers of antibody against 5- hydroxymethyl-2'-deoxyuridine (HMdU). Cancer Epidemiology Biomarkers and Prevention, 8(8), 693-698.