Abstract
This study evaluated the effects of vitamin E (α-tocopherol) on oxidative DNA damage in a randomized double-blind Phase II chemoprevention trial. Oxidative DNA damage was measured by the level of auto-antibody (Ab) against 5-hydroxymethyl-2'-deoxyuridine (HMdU) in plasma. After the baseline screening, eligible subjects (n = 31; plasma samples from 28 subjects were available for this study) were randomized to receive 15, 60, or 200 mg of α- tocopherol per day for 28 days. Biomarkers were measured twice at baseline - on day 1 (visit 1) and day 3 (visit 2) - and twice after intervention - on day 17 (visit 3) and day 31 (visit 4). At baseline, there was a highly significant inverse correlation between anti-HMdU Ab titer and plasma vitamin E level (r = -0.53; P = 0.004; n = 28). Smoking did not affect baseline anti- HMdU Ab titer; however, anti-HMdU Ab titer levels at baseline were significantly lower in subjects with above-median (0.75 ounce/day) alcohol consumption (P = 0.008). No significant change in anti-HMdU Ab level occurred at either visit 3 or visit 4 for subjects on the lowest dose, 15 mg α- tocopherol per day. Subjects receiving 60 mg of α-tocopherol per day had a significant decrease in anti-HMdU Ab level at visits 3 and 4 compared with baseline (P = 0.049 and P = 0.02, respectively). However, subjects receiving the highest dose, 200 mg/day, had less consistent results: a significant decrease in anti-HMdU Ab level was seen at visit 4 (P = 0.04) but not at visit 3. Our results demonstrate an inverse relationship between α- tocopherol and anti-HMdU Abs in plasma; oxidative DNA damage can be modulated by short-term dietary supplementation of α-tocopherol in some subjects.
| Original language | English |
|---|---|
| Pages (from-to) | 693-698 |
| Number of pages | 6 |
| Journal | Cancer Epidemiology Biomarkers and Prevention |
| Volume | 8 |
| Issue number | 8 |
| State | Published - Aug 1 1999 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Epidemiology
- Oncology
Cite this
α-Tocopherol dietary supplement decreases titers of antibody against 5- hydroxymethyl-2'-deoxyuridine (HMdU). / Hu, Jennifer; Chi, Chuan Xiang; Frenkel, Krystyna; Smith, Brian N.; Henfelt, John J.; Berwick, Marianne; Mahabir, Somdat; D'Agostino, Ralph B.
In: Cancer Epidemiology Biomarkers and Prevention, Vol. 8, No. 8, 01.08.1999, p. 693-698.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - α-Tocopherol dietary supplement decreases titers of antibody against 5- hydroxymethyl-2'-deoxyuridine (HMdU)
AU - Hu, Jennifer
AU - Chi, Chuan Xiang
AU - Frenkel, Krystyna
AU - Smith, Brian N.
AU - Henfelt, John J.
AU - Berwick, Marianne
AU - Mahabir, Somdat
AU - D'Agostino, Ralph B.
PY - 1999/8/1
Y1 - 1999/8/1
N2 - This study evaluated the effects of vitamin E (α-tocopherol) on oxidative DNA damage in a randomized double-blind Phase II chemoprevention trial. Oxidative DNA damage was measured by the level of auto-antibody (Ab) against 5-hydroxymethyl-2'-deoxyuridine (HMdU) in plasma. After the baseline screening, eligible subjects (n = 31; plasma samples from 28 subjects were available for this study) were randomized to receive 15, 60, or 200 mg of α- tocopherol per day for 28 days. Biomarkers were measured twice at baseline - on day 1 (visit 1) and day 3 (visit 2) - and twice after intervention - on day 17 (visit 3) and day 31 (visit 4). At baseline, there was a highly significant inverse correlation between anti-HMdU Ab titer and plasma vitamin E level (r = -0.53; P = 0.004; n = 28). Smoking did not affect baseline anti- HMdU Ab titer; however, anti-HMdU Ab titer levels at baseline were significantly lower in subjects with above-median (0.75 ounce/day) alcohol consumption (P = 0.008). No significant change in anti-HMdU Ab level occurred at either visit 3 or visit 4 for subjects on the lowest dose, 15 mg α- tocopherol per day. Subjects receiving 60 mg of α-tocopherol per day had a significant decrease in anti-HMdU Ab level at visits 3 and 4 compared with baseline (P = 0.049 and P = 0.02, respectively). However, subjects receiving the highest dose, 200 mg/day, had less consistent results: a significant decrease in anti-HMdU Ab level was seen at visit 4 (P = 0.04) but not at visit 3. Our results demonstrate an inverse relationship between α- tocopherol and anti-HMdU Abs in plasma; oxidative DNA damage can be modulated by short-term dietary supplementation of α-tocopherol in some subjects.
AB - This study evaluated the effects of vitamin E (α-tocopherol) on oxidative DNA damage in a randomized double-blind Phase II chemoprevention trial. Oxidative DNA damage was measured by the level of auto-antibody (Ab) against 5-hydroxymethyl-2'-deoxyuridine (HMdU) in plasma. After the baseline screening, eligible subjects (n = 31; plasma samples from 28 subjects were available for this study) were randomized to receive 15, 60, or 200 mg of α- tocopherol per day for 28 days. Biomarkers were measured twice at baseline - on day 1 (visit 1) and day 3 (visit 2) - and twice after intervention - on day 17 (visit 3) and day 31 (visit 4). At baseline, there was a highly significant inverse correlation between anti-HMdU Ab titer and plasma vitamin E level (r = -0.53; P = 0.004; n = 28). Smoking did not affect baseline anti- HMdU Ab titer; however, anti-HMdU Ab titer levels at baseline were significantly lower in subjects with above-median (0.75 ounce/day) alcohol consumption (P = 0.008). No significant change in anti-HMdU Ab level occurred at either visit 3 or visit 4 for subjects on the lowest dose, 15 mg α- tocopherol per day. Subjects receiving 60 mg of α-tocopherol per day had a significant decrease in anti-HMdU Ab level at visits 3 and 4 compared with baseline (P = 0.049 and P = 0.02, respectively). However, subjects receiving the highest dose, 200 mg/day, had less consistent results: a significant decrease in anti-HMdU Ab level was seen at visit 4 (P = 0.04) but not at visit 3. Our results demonstrate an inverse relationship between α- tocopherol and anti-HMdU Abs in plasma; oxidative DNA damage can be modulated by short-term dietary supplementation of α-tocopherol in some subjects.
UR - http://www.scopus.com/inward/record.url?scp=12944263829&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=12944263829&partnerID=8YFLogxK
M3 - Article
C2 - 10744129
AN - SCOPUS:12944263829
VL - 8
SP - 693
EP - 698
JO - Cancer Epidemiology Biomarkers and Prevention
JF - Cancer Epidemiology Biomarkers and Prevention
SN - 1055-9965
IS - 8
ER -