Abstract
Using a panel of monoclonal antibodies to several different cytoskeletal elements in primary cultures derived from newborn rat hearts we report that fibroblasts similar to cardiac-muscle cells expressed the α-actin isoform of smooth muscle cells. However, striated muscle α-actin or desmin antibodies did not stain cardiac fibroblasts but did stain cardiac-muscle cells. The α- smooth muscle actin distributed as a stress fiber and in a cross-striated pattern in cardiac muscle while fibroblasts showed exclusive stress fiber staining. These results suggest that connective tissue cells during development of the heart contain muscle-specific elements which may relate to the organ-specific contractile function with which they are associated.
Original language | English (US) |
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Pages (from-to) | 293-296 |
Number of pages | 4 |
Journal | In Vitro Cellular and Developmental Biology - Animal |
Volume | 28 A |
Issue number | 4 |
DOIs | |
State | Published - Jan 1 1992 |
Externally published | Yes |
ASJC Scopus subject areas
- Developmental Biology
- Clinical Biochemistry
- Cell Biology