α-Smooth muscle actin expression in cultured cardiac fibroblasts of newborn rat

D. Brouty-Boye, D. Kolonias, N. Savaraj, T. J. Lampidis

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Using a panel of monoclonal antibodies to several different cytoskeletal elements in primary cultures derived from newborn rat hearts we report that fibroblasts similar to cardiac-muscle cells expressed the α-actin isoform of smooth muscle cells. However, striated muscle α-actin or desmin antibodies did not stain cardiac fibroblasts but did stain cardiac-muscle cells. The α- smooth muscle actin distributed as a stress fiber and in a cross-striated pattern in cardiac muscle while fibroblasts showed exclusive stress fiber staining. These results suggest that connective tissue cells during development of the heart contain muscle-specific elements which may relate to the organ-specific contractile function with which they are associated.

Original languageEnglish (US)
Pages (from-to)293-296
Number of pages4
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume28 A
Issue number4
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

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