STUDIES ON HUMAN LAMBDA LIGHT CHAIN IMMUNOGLOBULIN GENES

Project: Research project

Description

The general objective of the proposed research is to establish molecular
mechanisms for the control of immunoglobulin (Ig) gene expression in human
normal and disease states. Specifically, the organization and regulation
of expression of the human lambda (Lambda) light chain V (variable), J
(joining) and C (constant) gene segments will be studied. Specific
objectives include the localization, isolation and characterization of
JLambda gene segments, isolation of DNA probes which will detect specific
VLambda subgroups and Clambda isotypes, and cloning and sequencing germline
and rearranged (active) Vlambda gene segments. This project will give
information on the molecular organization of the human Lambda genes, the
degree and location of somatic diversification in the VLambda gene
segments, which JLambda and CLambda gene segments are functional and allow
molecular study of preferential V-J-C association. It will provide a basis
for a molecular study of preferential light chain expression (Lambda) in
particular autoimmune diseases and the preferential use of the Lambda light
chain with certain heavy chain classes. An additional specific objective
is to assay for transcription enhancer elements associated with the above
Ig genes. For these objectives we will clone rearranged and germline Ig
Lambda genes by recombinant DNA techniques, insert these genes into plasmid
vectors which have positive selection markers (Ecogpt or cat), transfect
various lymphoid cells with these or deleted mutants, study expression of
these genes by cellular immunofluorescence and electrophoresis of proteins
and nucleic acids, and sequence particular DNA fragments by established
procedures.
StatusFinished
Effective start/end date12/1/844/30/96

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $144,203.00
  • National Institutes of Health
  • National Institutes of Health: $18,480.00
  • National Institutes of Health: $125,334.00
  • National Institutes of Health

Fingerprint

Immunoglobulin lambda-Chains
Immunoglobulin Light Chain Genes
Genes
Light
Gene Expression
Immunoglobulin Genes
Gene Rearrangement
Hybridomas
Myeloma Proteins

ASJC

  • Medicine(all)
  • Immunology and Microbiology(all)