PLASTICITY AND NEUROPATHOLOGY OF PAIN AND TRANSPLANTS

Project: Research project

Project Details

Description

DESCRIPTION: (Applicant's abstract) Studies in this laboratory and
elsewhere have shown that transplants of adrenal chromaffin cells into the
spinal subarachnoid space can result in reduction in "pain" behaviors in rat
models of persistent pain. In a continuation of this work, experiments are
proposed using rats with persistent pain due to experimentally-induced
arthritis or sciatic nerve constriction. Two primary goals will be
addressed; these are: 1) to assess the safety of adrenal medullary and
chromaffin cell transplants in the spinal subarachnoid space; and 2) to
clarify the mechanisms of reduction of pain behaviors consequent to adrenal
medullary transplants, with particular attention to analysis of the effects
on neuroplastic remodeling and long-term neuropathological consequences of
chronic pain. The "safety" issue will be addressed by analysis of three
factors: 1) motor and other physiological functions; 2) cerebrospinal fluid
and blood serum, and 3) neuropathology in spinal cord tissues. The effects
of adrenal medullary transplants on functional plasticity in the central
nervous system will be examined in the animal pain models with a combination
of behavioral measurements and measurements of immediate early gene
expression in spinal and supraspinal structures. Evaluation of the benefits
of transplants on long-term pathological processes will be examined
histologically by analysis of spinal tissues for the presence of
hyperchromatic ("dark") neurons and for changes in the numbers of neurons
expressing the inhibitory neurotransmitter GABA.
StatusFinished
Effective start/end date1/1/908/31/01

Funding

  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke

Fingerprint

Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.