NEUROCHEMISTRY OF THE DIENCEPHALON

Project: Research project

Description

The objective of the proposed research is the continued investigation of
the role the hypothalamic peptides play in the regulation of secretion of
the anterior pituitary gland and in control of other bodily functions. The
goals of this work include: 1. The isolation from porcine hypothalamic tissue of
Prolactin-Release-Inhibiting Hormone (PIF), Prolactin Releasing Hormone
(PRH), an antimitogenic (growth inhibiting) factor, larger and more basic
forms of CRF and other peptides with biological activities. 2. The determination of structure of these peptides. 3. The synthesis of these neurohormones and their analogs. Some peptide
fragments will be also synthesized in order to determine the specificity of
our RIA's for CRF and GH-RF. 4. Comparison of biological, chemical and immunological properties of
porcine hypothalamic hormones with those of other species and location of
structural differences, and determination of structure-activity
relationship for various hypothalamic hormones. 5. Generation of antisera to these peptides for the development of
radioimmunoassays and immunocytochemical localization studies. 6. Extensive physiological and biochemical studies on natural hypothalamic
hormones, synthetic hormones and their synthetic analogs, including
investigation of half life, metabolism and inactivation. These studies
will be important for planning realistic clinical tests. An investigation
of the effects of chronic administration of hypothalamic hormones on some
endocrine dependent or hormone sensitive tumors will be also carried out. 7. Continued search for a follicle stimulating hormone-releasing hormone
(FSH-RH) distinct from the decapeptide LH-RH (Gn-RH). 8. The investigation of the effect of hypothalamic peptides on lipid
mobilization, appetite and gastric acid secretion; purification, isolation,
determination of structure and synthesis of some of these peptides. 9. Providing of detailed information and ideas to clinicians on natural and
synthetic materials for the planning of clinical tests on human subjects.
StatusFinished
Effective start/end date12/1/746/30/88

Funding

  • National Institutes of Health

Fingerprint

Hypothalamic Hormones
Peptides
Hormones
Gonadotropin-Releasing Hormone
Prolactin-Releasing Hormone
Planning
Follicle Stimulating Hormone
Bioactivity
Metabolism
Purification
Neurotransmitter Agents
Neurochemistry
Tumors
Immune Sera
Tissue
Acids

ASJC

  • Medicine(all)