NEURAL PLATE DIFFERENTIATION AND TRANSFORMATION

Project: Research project

Project Details

Description

Cellular differentiation will be studied in neural plate cells of the early
chick embryo. Differentiation of neural cells will be identified by
indirect immunofluorescence staining using cell-type-specific antibody
probes for neurons, astrocytes, oligodendrocytes and fibroblasts. Studies
will be carried out to locate precursor cells in neural plate and map
regions which exhibit morphogenetic activity for parts of the neural system
of the embryo. The "determined" state of neural plate cells will be
altered by subjecting cells to various dissociation and grafting
techniques. These investigations will test the stability of the
"determined" state and examined the preceding events in its establishment.
Other experiments will establish whether the neural plate is composed of
subpopulations of committed cells of non-overlapping developmental fates or
whether they are "pluripotent". The approach will be to isolate neural
plate cells and transform the cells with a temperature-sensitive mutant of
Rous sarcoma virus, tsNY68, to inhibit differentiation and then to utilize
the property of transformation which allows cloning of individual cells.
The tsNY68 transformants will be grown to large number and then shifted to
the nonpermissive temperature to allow the clones to express their
developmental potential. The long-range goal of this project is to analyze
molecular mechanisms which regulate early neural differentiation. Such
information will be of importance in understanding control mechanisms
operating in malignancy.
StatusFinished
Effective start/end date12/31/891/1/90

Funding

  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke

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