MONOCLONAL ANTIBODIES AGAINST THE DEVELOPING RETINA

Project: Research project

Description

The neural retina develops from an epithelia of undifferentiated cells into
a stratified structure with a highly organized appearance and several
different types of cells. While there is a great deal of morphological and
physiological information about the mature retina, very little is known
about the cellular interactions and biochemical events that result in the
retina's normal development. One of the major reasons for this is that
techniques that allow identification of specific cell types and the
purification of these populations have not been available. As a result,
many in vivo and in vitro experiments designed to address developmental
quesitons are presently impossible to perform or interpret. We have
developed a number of monoclonal antibodies that bind to specific classes
of cells in the chick retina, including ganglion cells, amacrine cells,
photoreceptors, and Muller cells. One of our goals is characterize the
cell specific antigens defined by these antibodies using both morphological
and biochemical techniques. We also propose to use recently develop0ed
approaches to produce antibodies specific for bipolar cells and horizontal
cells. Finally, these antibodies will be used in in vitro experiments to
study how cell-cell interactions and various types of substrates ionfluence
the differentiation of specific classes of cells in the retina.
StatusFinished
Effective start/end date7/1/836/30/05

Funding

  • National Institutes of Health: $251,235.00
  • National Institutes of Health
  • National Institutes of Health: $332,325.00
  • National Institutes of Health: $237,357.00
  • National Institutes of Health: $303,000.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $250,555.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $219,748.00
  • National Institutes of Health: $258,772.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $132,182.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $303,000.00
  • National Institutes of Health

Fingerprint

Retinal Ganglion Cells
Axons
Cell Adhesion Molecules
Retina
Second Messenger Systems
Growth
Growth Cones
Monoclonal Antibodies
Cell Adhesion
Neural Cell Adhesion Molecule L1
Phosphorylation
Cell Communication
Optic Nerve
Site-Directed Mutagenesis
Antibodies
Brain
Learning
Mutation
Complementary DNA
Ependymoglial Cells

ASJC

  • Medicine(all)