MOLECULAR BASIS FOR HERPESVIRUS SAIMIRI ONCOGENICITY

Project: Research project

Description

Unlike other model tumor virus systems, little is known at the molecular
level about any herpesvirus induced oncogenic transformation. Oncogenic
transformation of T cells by Herpesvirus saimiri is being studied to
elucidate the molecular details of the process by which a normal T cell is
converted into an abnormally growing cancer cell. The current studies will
expand upon our previous experiments which identified a region of the viral
genome that is required for the lymphoma inducing capacity of the virus;
this region of the genome is not required for replication of the virus. 1) The protein products derived from this region of the genome in
permissively infected cells will be identified and characterized. A small
RNA derived from this region of the genome in tumor cell lines and tumor
biopsy samples will be mapped, sequenced and characterized. 2) The in vitro transforming ability of wild type Herpesvirus saimiri will
be compared to that of a series of mutants with constructed deletions in
this region. The design of the deletion derivatives is such that each will
be expected to affect the synthesis of only one or two of the four RNAs
derived from this region. Changes in the synthesis of RNA and protein
products by non-transforming deletion derivatives will be examined. 3) Non-pathogenic strains of Herpesvirus saimiri will be constructed that
contain genes for bovine growth hormone and human apo A-1 lipoprotein and
that express these genes in vitro and in vaccinated New World primates.
These experiments are aimed at demonstrating the utility of herpesvirus
vaccines for gene therapy in an animal model.
StatusFinished
Effective start/end date3/1/827/31/16

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $167,166.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

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Saimiriine Herpesvirus 2
Genome
T-Lymphocytes
Oncogenic Viruses
Herpesviridae
Apolipoprotein A-I
Virus Replication
Tumor Cell Line
Genetic Therapy
Primates
Genes
Lipoproteins
Lymphoma
Animal Models
RNA
Viruses
Neoplasms
Proteins
In Vitro Techniques

ASJC

  • Medicine(all)