MICROENVIRONMENT CONTROL OF NEURON PROPERTIES

Project: Research project

Description

We are trying to unravel the mechanisms which control the functional properties
of spinal cord neurons. We have found that muscle cells grown in culture
produce a high molecular weight substance which can enhance the growth of the
axons of spinal cord neurons. In this coming year we plan to make more accurate
measurements of the chemical properties of this trophic substance. We will use
gel filtration and ultracentrifuge sedimentation techniques to obtain measures
of its molecular weight. Isoelectric focusing and ion exchange chromotography
will be used in determining its isoelectric point. Using these techniques
together with affinity chromotagraphy methods we will continue to improve
purification methods for this trophic molecule. We will also try to elucidate the mechanism of activation of the slow potassium
conductance system found in motoneurons and muscle cells. This year we hope to
determine how membrane depolarization and internal calcium contribute to the
activation of this conductance system.
StatusFinished
Effective start/end date7/1/783/31/05

Funding

  • National Institutes of Health: $204,629.00
  • National Institutes of Health: $333,450.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $337,500.00
  • National Institutes of Health: $337,500.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $337,500.00
  • National Institutes of Health: $133,745.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $242,730.00

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Cholinergic Neurons
Dopaminergic Neurons
Nerve Growth Factors
Neurons
Primates
Hypoglycemic Agents
Phosphotransferases
Muscle Cells
Nerve Growth Factor
Spinal Cord
Blocking Antibodies
Molecular Weight
Choline O-Acetyltransferase
Serum
Ion Exchange
Membranes
Isoelectric Point
Isoelectric Focusing
Motor Neurons
Aptitude

ASJC

  • Medicine(all)
  • Neuroscience(all)