IDENTIFICATION OF THE MOUSE POLYADENYLATION SIGNAL

Project: Research project

Description

This research proposal is concerned with the process of polyadenylation in
mouse. The specific objectives of the proposed research are: (a)
Identification of the DNA sequences specifying the site of polyadenylation,
(the polyadenylation signal) and (b) Reexamination of the requirement for
polyadenylation in the biogenesis of mRNA, in vies of recent observations
from our lab (see Preliminary Results). The experimental approach
comprises in vitro reconstitution of genes, reintroduction into the mouse
chromosomes by transfection and analysis of the corresponding RNA species.
The high measure of flexibility afforded by in vitro gene reconstitution
experiments is considered to confer a major advantage to a successful
completion of this research proposal.
StatusFinished
Effective start/end date1/1/8312/31/85

Funding

  • National Institutes of Health

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Polyadenylation
Research Design
Genes
Transfection
RNA
Messenger RNA
Research
In Vitro Techniques

ASJC

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)