FORMATION OF CELL-CELL CHANNELS

Project: Research project

Description

Cells in most tissues are interconnected by cell-cell channels which
allow the passage of electrolytes and small molecules from cell to cell.
Aggregates of these channels are contained in the clustered
intramembranous particles of gap junctions. The function of these
channels has remained mysterious except in excitable cells where they are
instrumental for impulse propagation. The objective of this research project is to understand the process of
formation of cell-cell channels. This problem is addressed by functional
expression of cell-cell channels in paired oocytes from cloned gap
junction cDNA. With the oocyte assay conditions can be chosen where
channel formation occurs at high rate (greater than 40 channel openings
per second) from a pool of precursor. The oocyte assay will be used in
combination with mutagenesis, synthetic peptides and the patch clamp
technique to obtain information about molecular domains involved in the
formation process. Channel formation at another level is proposed to be studied with gene
transfer into mice (transgenics). These experiments are designed to
yield information about the expression patterns of specific gap junction
proteins during embryonic development. Furthermore, clues about the
function of cell-cell channels in nonexcitable tissues can be expected
from specific interference with gene expression.
StatusFinished
Effective start/end date1/1/9311/30/11

Funding

  • National Institutes of Health: $326,429.00
  • National Institutes of Health: $316,271.00
  • National Institutes of Health
  • National Institutes of Health: $244,800.00
  • National Institutes of Health: $309,139.00
  • National Institutes of Health
  • National Institutes of Health: $268,501.00
  • National Institutes of Health: $213,055.00
  • National Institutes of Health: $260,710.00
  • National Institutes of Health: $224,924.00
  • National Institutes of Health: $323,013.00
  • National Institutes of Health
  • National Institutes of Health: $316,212.00
  • National Institutes of Health: $326,354.00
  • National Institutes of Health: $325,976.00
  • National Institutes of Health: $316,328.00

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Connexins
Gap Junctions
cells
gametocytes
Oocytes
Calcium
electric potential
Proteins
Extracellular Space
Calcium Channels
Research
Cell Communication
tracers
molecules
Vertebrates
Permeability
Cytoplasm
Electrolytes
porosity
mutagenesis

ASJC

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)