EXTRACELLULAR MATRIX IN GLAUCOMA

Project: Research project

Description

Previous ultrastuctural and histochemical investigations by others have
provided qualitative evidence suggesting that the outflow obstruction in
primary open-angle glaucoma (POAG) is due to abnormal accumulation of
extracellular matrix materials in the trabecular meshwork. New
micromethods developed in our laboratories now allow us to 1) biochemically
measure and characterize the glycosaminoglycans (GAGs) of a single human
trabecular meshwork, and 2) to perform quantitative histochemical analysis
of the GAGs in specific regions of the meshwork. With these new tools, we
propose to study the GAG composition and localization in human trabecular
meshworks from eyebank eyes of POAG donors and age-matched controls,
obtained from a national registry and our own donor program. The
histochemical method will also be performed upon surgical specimens
obtained from POAG eyes and eyes with secondary open-angle glaucomas
(treatment controls). In companion studies, the trabecular meshwork from the opposite eyes of
POAG and normal donors will be placed in organ and/or cell culture, and the
profile of radiolabeled GAG systhesis and turnover determined. The goal of
the culture work will be to determine whether any abnormalities in GAG
content or composition demonstrated in vivo are expressed in vitro, so that
they might be traced to underlying biochemical mechanisms.
StatusActive
Effective start/end date7/1/86 → …

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

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Glycosaminoglycans
Glaucoma
Extracellular Matrix
Trabecular Meshwork
Open Angle Glaucoma
Organ Culture Techniques
Registries
Cell Culture Techniques

ASJC

  • Medicine(all)