Development and Characterization of Guided Optic Nerve Regeneration

Project: Research project

Description

? DESCRIPTION (provided by applicant): Poor regeneration and reconnection of retinal ganglion cell (RGC) axons is a major obstacle for treating ocular trauma and diseases including glaucoma. There are as yet no therapies to repair optic nerve once the damage is done. Our new studies now demonstrate that simultaneous manipulation of multiple molecules specifically in RGCs using adeno-associated virus vectors promotes long distance optic nerve regeneration in mice. Furthermore, we now demonstrate improved axon pathfinding and re-innervation of RGC axons using local expression of chemoattractants. In Aim 1, we will use transgenic mice and electron microscopy to examine whether these axons in fact form synapses. In Aim 2, we will examine strategies to further improve target re-innervation. In Aim 3, we will examine whether the manipulated RGCs show normal or altered physiology. Identifying methods to further increase regeneration to brain and determining whether the regenerated axons restore visual functions represent critical future studies. Results obtained from these studies will provid invaluable information on developing future therapies to repair degenerated optic nerve.
StatusFinished
Effective start/end date4/1/163/31/18

Funding

  • National Institutes of Health: $230,250.00
  • National Institutes of Health: $191,875.00

Fingerprint

Nerve Regeneration
Optic Nerve
Axons
Retinal Ganglion Cells
Regeneration
Dependovirus
Eye Diseases
Chemotactic Factors
Glaucoma
Synapses
Transgenic Mice
Electron Microscopy
Wounds and Injuries
Brain
Therapeutics