CONTROL OF VIRULENCE PROTEIN EXPORT IN YERSINIA PESTIS

Project: Research project

Project Details

Description

DESCRIPTION (Adapted from applicant's abstract): This project will
analyze the function of the LcrE protein in Yersinia pestis. LcrE is
implicated in the regulation of a unique secretion mechanism for
virulence proteins, the Yop proteins of Yersinia. The Yops lack
identifiable signal sequences and are secreted without proteolytic
processing. Expression and secretion of Yops in vitro regulated by
calcium. Eucaryotic cells triggers the translocation of at least one
Yop directly from the surface attached bacterium into the host cell.

This appears to be a mechanism of broad interest since it appears to be
present in other pathogens as well. This application proposes to
identify functional domains of LcrE required to target LcrE to the Yop
secretion apparatus, identify domains required to regulate Yop
secretion, and identify proteins that interact with LcrE. This will be
accomplished by deletion analysis of LcrE, characterization of second-
site mutations to identify proteins that interact with LcrE. Protein
affinity chromatography, affinity blotting and immunoprecipitation will
be used to confirm these interactions. Finally, the effect of LcrE
mutants on the translocation of YopE into eucaryotic cells will be
determined. These experiments are designed to map regions and residues
of LcrE to specific functions and reveal how LcrE functions.
StatusFinished
Effective start/end date6/1/965/31/01

Funding

  • National Institute of Allergy and Infectious Diseases: $111,991.00
  • National Institute of Allergy and Infectious Diseases
  • National Institute of Allergy and Infectious Diseases
  • National Institute of Allergy and Infectious Diseases

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