CONTROL OF AXIAL MUSCLE DEVELOPMENT

Project: Research project

Project Details

Description

Determination, differentiation, and patterned morphogenesis are central
processes during development, but the mechanisms controlling them have yet
to be satisfactorily identified. These processes are accessible to
analysis in a simple vertebrate system, the development of axial muscle
from the epithelial dermatome in the chicken embryo. Pilot studies
suggest that the dermatome generates two cell phenotypes under a tight
spatial control. Furthermore, this generation pattern can be altered
experimentally. A simple surgery causes the dermacyte-forming population
to form myocytes instead. Moreover, the dermatome gives rise to axial
muscles that manifest different fates at limb and thoracic levels. For
instance, preliminary results show that programmed death of dermatome
prevents development of lateral axial muscles at limb levels and suggest
that this death is induced by an interaction with limb tissue.

Proposed studies focus on the spatial control of cell generation in this
system and the interactions controlling cell generation patterns and
muscle fate. Studies in five aims will 1) finely map the fate of
dermatome cells and characterize the development of muscles derived from
dermatome, 2) assess the pluripotency of spatially defined dermatomal
populations, 3) define interactions that control the patterned generation
of myocytes and dermacytes, 4) define interactions that control the
segment-specific fates of axial muscles and 5) assess the relevance of
putative regulatory genes by defining expression patterns and using
experimental manipulations. The approaches combine anatomical methods,
fate-mapping strategies and embryonic surgeries. This work will help
elucidate mechanisms that control the patterned generation of specific
cell types and the morphogenesis of muscles, processes that are
fundamental to normal human development.
StatusFinished
Effective start/end date12/21/9411/30/00

Funding

  • Eunice Kennedy Shriver National Institute of Child Health and Human Development
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development

Fingerprint

Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.