CONFOCAL LASER-SCANNING MICROSCOPE

Project: Research project

Description

An interdepartmental group of NIH-supported investigators seeks funds for a Perkin-Elmer Wallac Ultraview confocal microscope system, to replace and upgrade an existing Noran system that has several major deficiencies and is starting to fail. The requested system has several important advantages that will benefit many NIH-sponsored research programs at this institution. The noel design of this confocal system allows use of very low intensities of excitation light, thus minimizing phototoxicity and bleaching. The high sensitivity of the light-detection systems permits measurements of weaker signals than can be detected by our current confocal system. The requested system also has a much better data-collecting ability (14 bit sampling) than the existing system (8 bits). The application details why the requested confocal system is essential and/or important for optimal progress of NIH-sponsored projects of major users from five laboratories. Four of the laboratories will be imaging living tissues, and share research interests in mitochondrial function. All major users have a history of cooperative interactions, and one has access to any other suitable confocal facility. The confocal needs of five additional laboratories are briefly summarized. All major users attended a demonstration of the requested confocal system at the University of Miami, during which some of the tissues and fluorophores to be imaged were examined. In all cases the requested confocal system, facilitating experiments that are now difficult or impossible to perform.
StatusFinished
Effective start/end date5/1/027/31/04

Funding

  • National Institutes of Health: $235,410.00

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microscopes
scanning
lasers
bleaching
sampling
histories
sensitivity
excitation
interactions

ASJC

  • Medicine(all)