Conditional Knock Out Mouse Model for Neurofibromatosis

  • Bajenaru, Michaela, (PI)

Project: Research project

Description

DESCRIPTION: (As provided by Applicant)
This NRSA application targets one of the most important clinical issues in
neurofibromatosis (NFl). Astrocytomas are the second most common tumor in NF1,
often leading to blindness and neurologic impairment. Our ability to design
rational therapies for these tumors is heavily dependent on a more complete
understanding of the role of the Nfl gene in the development of astrocytomas.
In this grant, we propose to critically test the hypothesis that loss of
neurofibromin expression results in astrocytoma formation. Specifically, we
plan to (1) determine the effect of loss of neurofibromin expression in vivo
using a conditional knockout mouse in which Nfl gene expression has been
disrupted in astrocytes by using the Cre/Lox P recombination system and (2)
determine the effect of loss of neurofibromin expression in vitro by
inactivating the Nfl gene in primary astrocyte cultures. Astrocytes in culture
will be assessed for their growth and tumorigenic properties and since
neurofibromin is a negative regulator of p21-ras, downstream effectors of the
p21-ras signaling pathway will be measured.
StatusActive
Effective start/end date8/1/01 → …

Funding

  • National Institutes of Health: $46,192.00
  • National Institutes of Health: $40,196.00

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Neurofibromatoses
Astrocytoma
Neurofibromin 1
Knockout Mice
Astrocytes
Proto-Oncogene Proteins p21(ras)
Organized Financing
Blindness
Nervous System
Genetic Recombination
Genes
Neoplasms
Gene Expression
Growth
Therapeutics

ASJC

  • Medicine(all)