CANCER IN DAMSELFISH--IMMUNOLOGY AND IN VITRO MODELS

Project: Research project

Description

DESCRIPTION: The proposed study will
address two areas of research which are important in understanding the
etiology of cancers in finfish and shellfish. The first objective is the
investigation of the role of the immune system in the initiation and
progression of tumor in these animals. The second is the development and
use of in vitro techniques to monitor neoplastic transformation in a
specific class of cells, those derived from the embryonic neural crest,
that are involved in a wide variety of tumors in fishes. The proposed
studies will be based on a unique model system, damselfish
neurofibromatosis (DNF), a malignant, transmissible cancer involving tumors
in tissues derived from the embryonic neural crest, including
neurofibromas, schwannomas and chromatophoromas. The histopathology,
epizootiology and etiology of DNA are currently being investigated by this
laboratory. Model systems of peripheral nerve sheath tumors (such as
neurofibromas and schwannomas) using fishes are of special interest because
mammalian models of these tumors are rare. The use of cultures of these
cell types for the study of neoplastic transformation leading to such
cancers has not been adequately explored in fishes or in mammals. Proposed
studies include: 1) quantification of morphological, ultrastructural and
antigenic differences in vitro between neoplastic cells from fish with DNA
and their normal counterparts, 2) evaluation of alterations in cell surface
receptors associated with pigment migration in chromatophores in DNF, 3)
development of models of transformation based on the exposure of selected
neuroectodermal cell types to transmissible factors isolated from tumors as
well as selected chemical carcinogens. Preliminary studies have indicated
that profound changes in immune function are associated with the
progression of this disease and that an inflammatory infiltrate is
characteristic of both spontaneous and experimentally induced tumors.
Studies of the immunology of DNF will include: 1) an evaluation of changes
in several cellular and humoral immune responses as the disease progresses,
2) measurement of effects of immunosuppression on latency, growth rate and
histological features of experimentally induced tumors, 3) characterization
of the structural, histochemical and functional cell types composing
populations of tumor infiltrating leukocytes.
StatusFinished
Effective start/end date1/1/9112/31/94

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $166,740.00

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Allergy and Immunology
Neoplasms
Fishes
Neural Crest
Neurilemmoma
Chromatophores
Nerve Sheath Neoplasms
In Vitro Techniques
Shellfish
Humoral Immunity
Cellular Immunity
Carcinogens
Immunosuppression
Mammals
Immune System
Leukocytes
DNA
Growth
Research

ASJC

  • Medicine(all)