BIOLOGICAL EFFECTS OF BASIC CALCIUM PHOSPHATE CRYSTALS

Project: Research project

Description

These studies are designed to determine the pathogenesis of the
degenerative arthropathies associated with the deposition of
calcium pyrophosphate dihydrate or basic calcium phosphate
crystals in articular tissues with the eventual goal of developing a
rational means of preventing or reversing the consequences of
such deposition. This proposal focuses chiefly on the interaction
of living cells with crystals containing calcium. We shall examine how the observed biological effects such as
stimulation of mitogenesis, crystal dissolution, arachidonic acid
metabolism and proteases synthesis are related to each other. We
shall further define the mechanism of BCP crystal-induced
mitogenesis with special emphasis on early biochemical changes in
cells stimulated by crystals. The mechanism of BCP crystal
dissolution by cells will be explored. We are interested in possible
intracellular sequestration of Ca2+ resulting from crystal
dissolution and rate of Ca2+ efflux. Other related studies will include: level of somatomedin C in
joint fluids of arthritis patients; 2) clearance of BCP crystals
from human joints. Rabbits and mice will be used as a source for cells for cell
culture.
StatusFinished
Effective start/end date2/1/886/30/05

Funding

  • National Institutes of Health: $167,760.00
  • National Institutes of Health: $212,852.00
  • National Institutes of Health: $248,704.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $234,666.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $231,201.00
  • National Institutes of Health: $233,861.00
  • National Institutes of Health: $256,164.00
  • National Institutes of Health: $241,460.00
  • National Institutes of Health
  • National Institutes of Health: $233,004.00

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Joints
Calcium
Calcium Pyrophosphate
Matrix Metalloproteinases
Joint Diseases
Chondrocalcinosis
Serum Response Factor
Arthritis
Cell Membrane
Tissue Inhibitor of Metalloproteinase-2
Osteoarthritis
Insulin-Like Growth Factor I
Mitogen-Activated Protein Kinase 1
calcium phosphate
Peptide Hydrolases
Metalloproteases
Signal Transduction
Rabbits
Periarthritis
Prostaglandins

ASJC

  • Medicine(all)