Nevis L. Fregien &ndash; Research Output

2019

Electronic cigarettes enhance replication of mycobacterium abscessus in airway epithelial cells

Korukonda, A., Zhang, C., Rodriguez, L., Guerrero, A., Campos, M. A., Holt, G., Fregien, N. L., Conner, G. E. & Mirsaeidi, M., Jun 1 2019, In : American journal of respiratory cell and molecular biology. 60, 6, p. 717-719 3 p.

Research output: Contribution to journal › Letter

Mycobacterium

Tobacco Products

Epithelial Cells

Electronic Cigarettes

2017

4 Citations (Scopus)

Modulation of Wnt signaling is essential for the differentiation of ciliated epithelial cells in human airways

Schmid, A., Sailland, J., Novak, L., Baumlin, N., Fregien, N. L. & Salathe, M. A., Nov 1 2017, In : FEBS Letters. 591, 21, p. 3493-3506 14 p.

Research output: Contribution to journal › Letter

Catenins

Epithelial Cells

Modulation

Cell Count

Glycogen Synthase Kinase 3

2016

6 Citations (Scopus)

Gefitinib, an EGFR tyrosine kinase inhibitor, prevents smoke-mediated ciliated airway epithelial cell loss and promotes their recovery

Valencia-Gattas, M., Conner, G. E. & Fregien, N. L., Aug 1 2016, In : PLoS One. 11, 8, e0160216.

Research output: Contribution to journal › Article

smoke

Epidermal Growth Factor Receptor

Smoke

Protein-Tyrosine Kinases

tyrosine

4 Citations (Scopus)

IL-13 inhibits multicilin expression and ciliogenesis via janus kinase/signal transducer and activator of transcription independently of notch cleavage

Gerovac, B. J. & Fregien, N. L., Apr 1 2016, In : American Journal of Respiratory Cell and Molecular Biology. 54, 4, p. 554-561 8 p.

Research output: Contribution to journal › Article

Janus Kinases

Interleukin-13

Transcription

Transducers

Cell Differentiation

6 Citations (Scopus)

Optimal lentivirus production and cell culture conditions necessary to successfully transduce primary human bronchial epithelial cells

Baumlin-Schmid, N., Salathe, M. A. & Fregien, N. L., Jul 22 2016, In : Journal of Visualized Experiments. 2016, 113, e54176.

Research output: Contribution to journal › Article

Lentivirus

Cell culture

Cell Culture Techniques

Epithelial Cells

Transgenes

2014

14 Citations (Scopus)

A soluble adenylyl cyclase form targets to axonemes and rescues beat regulation in soluble adenylyl cyclase knockout mice

Chen, X., Baumlin, N., Buck, J., Levin, L. R., Fregien, N. L. & Salathe, M. A., Jan 1 2014, In : American Journal of Respiratory Cell and Molecular Biology. 51, 6, p. 750-760 11 p.

Research output: Contribution to journal › Article

Axoneme

Adenylyl Cyclases

Knockout Mice

Cilia

Epithelial Cells

19 Citations (Scopus)

Submersion and hypoxia inhibit ciliated cell differentiation in a Notch-dependent manner

Gerovac, B. J., Valencia, M., Baumlin, N., Salathe, M. A., Conner, G. E. & Fregien, N. L., Jan 1 2014, In : American Journal of Respiratory Cell and Molecular Biology. 51, 4, p. 516-525 10 p.

Research output: Contribution to journal › Article

Immersion

Cell Differentiation

Cells

Amyloid Precursor Protein Secretases

Gene expression

2011

15 Citations (Scopus)

Rapid nongenomic actions of inhaled corticosteroids on long-acting β ₂-agonist transport in the airway

Horvath, G., Mendes, E., Schmid, N., Schmid, A., Conner, G. E., Fregien, N. L., Salathe, M. A. & Wanner, A., Dec 1 2011, In : Pulmonary Pharmacology and Therapeutics. 24, 6, p. 654-659 6 p.

Research output: Contribution to journal › Article

Adrenal Cortex Hormones

Smooth Muscle Myocytes

Muscle

Cells

Bronchodilator Agents

2010

33 Citations (Scopus)

Decreased soluble adenylyl cyclase activity in cystic fibrosis is related to defective apical bicarbonate exchange and affects ciliary beat frequency regulation

Schmid, A., Sutto, Z., Schmid, N., Novak, L., Ivonnet, P., Horvath, G., Conner, G. E., Fregien, N. L. & Salathe, M. A., Sep 24 2010, In : Journal of Biological Chemistry. 285, 39, p. 29998-30007 10 p.

Research output: Contribution to journal › Article

Bicarbonates

Adenylyl Cyclases

Cystic Fibrosis

Cystic Fibrosis Transmembrane Conductance Regulator

Acidification

66 Citations (Scopus)

Reactive oxygen species and hyaluronidase 2 regulate airway epithelial hyaluronan fragmentation

Monzon, M. E., Fregien, N. L., Schmid, N., Falcon, N. S., Campos, M. A., Casalino-Matsuda, S. M. & Forteza, R. M., Aug 20 2010, In : Journal of Biological Chemistry. 285, 34, p. 26126-26134 9 p.

Research output: Contribution to journal › Article

Hyaluronoglucosaminidase

Hyaluronic Acid

Reactive Oxygen Species

Glycosylphosphatidylinositols

Epithelial Cells

2009

13 Citations (Scopus)

Molecular heterogeneity and alternative splicing of human lactoperoxidase

Fragoso, M. A., Torbati, A., Fregien, N. L. & Conner, G. E., Feb 1 2009, In : Archives of Biochemistry and Biophysics. 482, 1-2, p. 52-57 6 p.

Research output: Contribution to journal › Article

Lactoperoxidase

Alternative Splicing

Exons

Glycosylation

Messenger RNA

58 Citations (Scopus)

Oxidative epithelial host defense is regulated by infectious and inflammatory stimuli

Gattas, M. V., Forteza, R., Fragoso, M. A., Fregien, N. L., Salas, P. J., Salathe, M. A. & Conner, G. E., Nov 15 2009, In : Free Radical Biology and Medicine. 47, 10, p. 1450-1458 9 p.

Research output: Contribution to journal › Article

Lactoperoxidase

Messenger RNA

Flagellin

Pseudomonas aeruginosa

Small Interfering RNA

142 Citations (Scopus)

Pannexin 1 contributes to ATP release in airway epithelia

Ransford, G. A., Fregien, N. L., Qiu, F., Dahl, G., Conner, G. E. & Salathe, M. A., Nov 26 2009, In : American Journal of Respiratory Cell and Molecular Biology. 41, 5, p. 525-534 10 p.

Research output: Contribution to journal › Article

Epithelium

Adenosine Triphosphate

Epithelial Cells

Osmotic Pressure

Liquids

2008

51 Citations (Scopus)

Atypical protein kinase C (iota) activates ezrin in the apical domain of intestinal epithelial cells

Wald, F. A., Oriolo, A. S., Mashukova, A., Fregien, N. L., Langshaw, A. & Salas, P. J., Mar 1 2008, In : Journal of Cell Science. 121, 5, p. 644-654 11 p.

Research output: Contribution to journal › Article

Epithelial Cells

Phosphorylation

Cyclic AMP-Dependent Protein Kinases

Sf9 Cells

ezrin

29 Citations (Scopus)

Epigenetic regulation of HYAL-1 hyaluronidase expression: Identification of HYAL-1 promoter

Lokeshwar, V. B., Gomez, P., Kramer, M., Knapp, J., McCornack, M. A., Lopez, L. E., Fregien, N. L., Dhir, N., Scherer, S., Klumpp, D. J., Manoharan, M., Soloway, M. S. & Lokeshwar, B. L., Oct 24 2008, In : Journal of Biological Chemistry. 283, 43, p. 29215-29227 13 p.

Research output: Contribution to journal › Article

Hyaluronoglucosaminidase

Epigenomics

Transcription Initiation Site

Tissue

Methylation

2007

15 Citations (Scopus)

Calcium-mediated, purinergic stimulation and polarized localization of calcium-sensitive adenylyl cyclase isoforms in human airway epithelia

Nlend, M. C., Schmid, A., Sutto, Z., Ransford, G. A., Conner, G. E., Fregien, N. L. & Salathe, M. A., Jul 10 2007, In : FEBS Letters. 581, 17, p. 3241-3246 6 p.

Research output: Contribution to journal › Article

Adenylyl Cyclases

Protein Isoforms

Epithelium

Epithelial Cells

Calcium

7 Citations (Scopus)

Control of core 2 β1,6 N-acetylglucosaminyltransferase-I transcription by Sp1 in lymphocytes and epithelial cells

Falkenberg, V. R. & Fregien, N. L., Dec 1 2007, In : Glycoconjugate Journal. 24, 9, p. 511-519 9 p.

Research output: Contribution to journal › Article

Lymphocytes

Transcription

Epithelial Cells

Mucins

Binding Sites

72 Citations (Scopus)

Soluble adenylyl cyclase is localized to cilia and contributes to ciliary beat frequency regulation via production of cAMP

Schmid, A., Sutto, Z., Nlend, M. C., Horvath, G., Schmid, N., Buck, J., Levin, L. R., Conner, G. E., Fregien, N. L. & Salathe, M. A., Jul 1 2007, In : Journal of General Physiology. 130, 1, p. 99-109 11 p.

Research output: Contribution to journal › Article

Cilia

Adenylyl Cyclases

Fluorescent Antibody Technique

Western Blotting

Axoneme

2006

52 Citations (Scopus)

Real-time analysis of cAMP-mediated regulation of ciliary motility in single primary human airway epithelial cells

Schmid, A., Bai, G., Schmid, N., Zaccolo, M., Ostrowski, L. E., Conner, G. E., Fregien, N. L. & Salathe, M. A., Oct 15 2006, In : Journal of Cell Science. 119, 20, p. 4176-4186 11 p.

Research output: Contribution to journal › Article

Cyclic AMP

Epithelial Cells

Fluorescence Resonance Energy Transfer

Phosphotransferases

Phosphoprotein Phosphatases

2005

26 Citations (Scopus)

Forskolin increases neuregulin receptors in human Schwann cells without increasing receptor mRNA

Fregien, N. L., White, L. A., Bunge, M. B. & Wood, P., Jan 1 2005, In : GLIA. 49, 1, p. 24-35 12 p.

Research output: Contribution to journal › Article

Neuregulins

Schwann Cells

Colforsin

Neuregulin-1

Messenger RNA

2003

9 Citations (Scopus)

Influenza a virus infection of human Schwann cells in vitro

Levine, J., Buchman, C. A. & Fregien, N. L., Feb 27 2003, In : Acta Oto-Laryngologica. 123, 1, p. 41-45 5 p.

Research output: Contribution to journal › Article

Schwann Cells

Influenza A virus

Virus Diseases

Orthomyxoviridae

Human Influenza

11 Citations (Scopus)

Multiple transcription initiation and alternative splicing in the 5′ untranslated region of the core 2 β1-6 N-acetylglucosaminyltransferase I gene

Falkenberg, V. R., Alvarez, K., Roman, C. & Fregien, N. L., Jun 1 2003, In : Glycobiology. 13, 6, p. 411-418 8 p.

Research output: Contribution to journal › Article

5' Untranslated Regions

Alternative Splicing

Transcription

Exons

Genes

2002

5 Citations (Scopus)

Sequences of the mouse N-acetylglucosaminyltransferase V (Mgat5) mRNA and an mRNA expressed by an Mgat5-deficient cell line

Alverez, K., Haswell, C., St. Clair, M., Perng, G. S., Shorebah, M., Pierce, M. & Fregien, N. L., Jul 1 2002, In : Glycobiology. 12, 7, p. 389-394 6 p.

Research output: Contribution to journal › Article

alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase

Cells

Cell Line

Messenger RNA

Genes

2000

10 Citations (Scopus)

Influenza A virus infection of human middle ear cells in vitro

Buchman, C. A. & Fregien, N. L., Jan 1 2000, In : Laryngoscope. 110, 10 I, p. 1739-1744 6 p.

Research output: Contribution to journal › Article

Influenza A virus

Middle Ear

Virus Diseases

Reverse Transcriptase Polymerase Chain Reaction

Keratin-18

1999

154 Citations (Scopus)

An intramembrane modulator of the ErbB2 receptor tyrosine kinase that potentiates neuregulin signaling

Carraway, K. L., Rossi, E. A., Komatsu, M., Price-Schiavi, S. A., Huang, D., Guy, P. M., Carvajal, M. E., Fregien, N. L., Carothers Carraway, C. A. & Carraway, K. L., Feb 26 1999, In : Journal of Biological Chemistry. 274, 9, p. 5263-5266 4 p.

Research output: Contribution to journal › Article

Neuregulins

Receptor Protein-Tyrosine Kinases

Modulators

Rats

Tumors

1998

54 Citations (Scopus)

Characterization of the expression and steroid hormone control of sialomucin complex in the rat uterus: Implications for uterine receptivity

McNeer, R., Carothers Carraway, C. A., Fregien, N. L. & Carraway, K. L., Jul 1 1998, In : Journal of Cellular Physiology. 176, 1, p. 110-119 10 p.

Research output: Contribution to journal › Article

Sialomucins

Steroid hormones

Uterus

Rats

Steroids

49 Citations (Scopus)

Post-transcriptional regulation of a milk membrane protein, the sialomucin complex (ascites sialoglycoprotein (ASGP)-1/ASGP-2, Rat Muc4), by transforming growth factor β

Price-Schiavi, S. A., Carothers Carraway, C. A., Fregien, N. L. & Carraway, K. L., Dec 25 1998, In : Journal of Biological Chemistry. 273, 52, p. 35228-35237 10 p.

Research output: Contribution to journal › Article

Mucin-4

Sialomucins

Milk Proteins

Transforming Growth Factors

Rats

45 Citations (Scopus)

Sialomucin complex in the rat respiratory tract: A model for its role in epithelial protection

McNeer, R., Huang, D., Fregien, N. L. & Carraway, K. L., Mar 1 1998, In : Biochemical Journal. 330, 2, p. 737-744 8 p.

Research output: Contribution to journal › Article

Sialomucins

Respiratory System

Rats

Epithelium

Epithelial Cells

82 Citations (Scopus)

The her-2/neu oncogene stimulates the transcription of N-acetylglucosaminyltransferase V and expression of its cell surface oligosaccharide products

Chen, L., Zhang, W., Fregien, N. L. & Pierce, M., Oct 22 1998, In : Oncogene. 17, 16, p. 2087-2093 7 p.

Research output: Contribution to journal › Article

alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase

Oligosaccharides

Oncogenes

Glycoproteins

Proto-Oncogene Proteins c-ets

1997

Characterization and steroid hormone control of sialomucin complex in rat uterus: Implications for uterine receptivity

McNeer, R., Carraway, C. A. C., Fregien, N. L. & Carraway, K. L., Dec 1 1997, In : Protein Engineering. 10, 3333

Research output: Contribution to journal › Article

Sialomucins

Steroid hormones

Rats

61 Citations (Scopus)

Regulation of N-acetylglucosaminyltransferase V and Asn-linked oligosaccharide β(1,6) branching by a growth factor signaling pathway and effects on cell adhesion and metastatic potential

Pierce, M., Buckhaults, P., Chen, L. & Fregien, N. L., Sep 3 1997, In : Glycoconjugate Journal. 14, 5, p. 623-630 8 p.

Research output: Contribution to journal › Article

alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase

N-Acetylglucosaminyltransferases

Glycosyltransferases

Growth Factor Receptors

Cell adhesion

Regulation of sialomucin complex expression in normal rat mammary epithelial cells

Price-Schiavi, S. A., Fregien, N. L., Carraway, C. A. C. & Carraway, K. L., Dec 1 1997, In : Protein Engineering. 10, 3333

Research output: Contribution to journal › Article

Sialomucins

Rats

Epithelial Cells

141 Citations (Scopus)

Reversible disruption of cell-matrix and cell-cell interactions by overexpression of sialomucin complex

Komatsu, M., Carothers Carraway, C. A., Fregien, N. L. & Carraway, K. L., Dec 26 1997, In : Journal of Biological Chemistry. 272, 52, p. 33245-33254 10 p.

Research output: Contribution to journal › Article

Sialomucins

Cell Communication

Mucin-4

Mucins

Cell adhesion

Sialomucin complex: A new respiratory mucin with potential growth factor activity is expressed as soluble and transmembrane forms in rat airways

McNeer, R., Huang, D., Carraway, C. A. C., Fregien, N. L. & Carraway, K. L., Dec 1 1997, In : Protein Engineering. 10, 3333

Research output: Contribution to journal › Article

Sialomucins

Mucins

Intercellular Signaling Peptides and Proteins

Sialomucin complex is secreted by proteolytic cleavage of membrane precursor

Komatsu, M., Rossi, E. A., McNeer, R., Carraway, C. A. C., Fregien, N. L. & Carraway, K. L., Dec 1 1997, In : Protein Engineering. 10, 3333

Research output: Contribution to journal › Article

Sialomucins

Membranes

108 Citations (Scopus)

Transcriptional regulation of N-acetylglucosaminyltransferase V by the src oncogene

Buckhaults, P., Chen, L., Fregien, N. L. & Pierce, M., Aug 1 1997, In : Journal of Biological Chemistry. 272, 31, p. 19575-19581 7 p.

Research output: Contribution to journal › Article

alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase

src Genes

src-Family Kinases

Messenger RNA

Transfection

Transmembrane glycoprotein ASGP

Carraway, K., Carothers, C. C. A. & Fregien, N. L., Jan 1 1997, In : Biotechnology Advances. 15, 3-4

Research output: Contribution to journal › Article

Glycoproteins

DNA

Recombinant DNA

Patents and inventions

Mucin-4

1996

103 Citations (Scopus)

Sialomucin complex, a heterodimeric glycoprotein complex: Expression as a soluble, secretable form in lactating mammary gland and colon

Rossi, E. A., McNeer, R., Price-Schiavi, S. A., Van den Brande, J. M. H., Komatsu, M., Thompson, J. F., Carothers Carraway, C. A., Fregien, N. L. & Carraway, K. L., Dec 1 1996, In : Journal of Biological Chemistry. 271, 52, p. 33476-33485 10 p.

Research output: Contribution to journal › Article

Mucin-4

Sialomucins

Human Mammary Glands

Glycoproteins

Colon

1995

36 Citations (Scopus)

GlcNAc-transferase V and core 2 GlcNAc-transferase expression in the developing mouse embryo

Granovsky, M., Fode, C., Warren, C. E., Campbell, R. M., Marth, J. D., Pierce, M., Fregien, N. L. & Dennis, J. W., Dec 1 1995, In : Glycobiology. 5, 8, p. 797-806 10 p.

Research output: Contribution to journal › Article

alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase

Embryonic Structures

Uridine Diphosphate

Epithelium

Biosynthesis

30 Citations (Scopus)

Mucin Structure and Function: Insights from Molecular Biology

Carraway, K. L. & Fregien, N. L., Jan 1 1995, In : Trends in Glycoscience and Glycotechnology. 7, 33, p. 31-44 14 p.

Research output: Contribution to journal › Article

Molecular biology

Mucins

Molecular Biology

Tissue

Cysteine

1994

185 Citations (Scopus)

Ankyrin-binding domain of CD44(GP85) is required for the expression of hyaluronic acid-mediated adhesion function

Lokeshwar, V. B., Fregien, N. L. & Bourguignon, L. Y. W., Aug 1 1994, In : Journal of Cell Biology. 126, 4, p. 1099-1109 11 p.

Research output: Contribution to journal › Article

Ankyrins

Hyaluronic Acid

Proteins

COS Cells

Cell Adhesion Molecules

29 Citations (Scopus)

Expression of n-acetylglucosaminyltransferase V mRNA in mammalian tissues and cell lines

Perug, G. S., Shoreibah, M., Margitich, I., Pierce, M. & Fregien, N. L., Dec 1 1994, In : Glycobiology. 4, 6, p. 867-871 5 p.

Research output: Contribution to journal › Article

Messenger RNA

Cells

Tissue

Cell Line

Line

33 Citations (Scopus)

Modification of glycoproteins by N-acetylglucosaminyltransferase V is greatly influenced by accessibility of the enzyme to oligosaccharide acceptors

Do, K. Y., Fregien, N. L., Pierce, M. & Cummings, R. D., Sep 23 1994, In : Journal of Biological Chemistry. 269, 38, p. 23456-23464 9 p.

Research output: Contribution to journal › Article

alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase

Oligosaccharides

Glycoproteins

Enzymes

Fetuins

13 Citations (Scopus)

Molecular Cloning and Sequencing of a 58-kDa Membrane- and Microfilament-associated Protein from Ascites Tumor Cell Microvilli with Sequence Similarities to Retroviral Gag Proteins

Juang, S. H., Huang, J., Li, Y., Salas, P. J., Fregien, N. L., Carothers Carraway, C. A. & Carraway, K. L., May 27 1994, In : Journal of Biological Chemistry. 269, 21, p. 15067-15075 9 p.

Research output: Contribution to journal › Article

gag Gene Products

Microfilament Proteins

Cloning

Molecular Cloning

Microvilli

64 Citations (Scopus)

Molecular cloning and sequencing of the mucin subunit of a heterodimeric, bifunctional cell surface glycoprotein complex of ascites rat mammary adenocarcinoma cells

Wu, K., Fregien, N. L. & Carraway, K. L., Apr 22 1994, In : Journal of Biological Chemistry. 269, 16, p. 11950-11955 6 p.

Research output: Contribution to journal › Article

Mucin-4

Cloning

Membrane Glycoproteins

Molecular Cloning

Mucins

19 Citations (Scopus)

Tissue and tumor expression of a cell surface glycoprotein complex containing an integral membrane glycoprotein activator of p185^neu

Wu, K., Salas, P. J., Yee, L., Fregien, N. L. & Carraway, K. L., Nov 1 1994, In : Oncogene. 9, 11, p. 3139-3147 9 p.

Research output: Contribution to journal › Article

Membrane Glycoproteins

Ascites

Neoplasms

Brain

Sialomucins

1993

57 Citations (Scopus)

Expression of the protein zero myelin gene in axon-related Schwann cells is linked to basal lamina formation

Fernandez-Valle, C., Fregien, N. L., Wood, P. & Bunge, M. B., Nov 1 1993, In : Development. 119, 3, p. 867-880 14 p.

Research output: Contribution to journal › Article

Myelin P0 Protein

Schwann Cells

Basement Membrane

Axons

Genes

110 Citations (Scopus)

Isolation, characterization, and expression of a cDNA encoding n-acetylglucosaminyltransferase V

Shoreibah, M., Perng, G. S., Adler, B., Weinstein, J., Basu, R., Cupples, R., Wen, D., Browne, J. K., Buckhaults, P., Fregien, N. L. & Pierce, M., Jul 25 1993, In : Journal of Biological Chemistry. 268, 21, p. 15381-15385 5 p.

Research output: Contribution to journal › Article

alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase

Complementary DNA

Rats

Clone Cells

COS Cells

1992

106 Citations (Scopus)

Molecular cloning of the transmembrane component of the 13762 mammary adenocarcinoma sialomucin complex: A new member of the epidermal growth factor superfamily

Sheng, Z., Wu, K., Carraway, K. L. & Fregien, N. L., Aug 15 1992, In : Journal of Biological Chemistry. 267, 23, p. 16341-16346 6 p.

Research output: Contribution to journal › Article

Mucin-4

Sialomucins

Cloning

Molecular Cloning

Epidermal Growth Factor

70 Citations (Scopus)

Tumor sialomucin complexes as tumor antigens and modulators of cellular interactions and proliferation

Carraway, K. L., Fregien, N. L., Carraway, K. L. & Carraway, C. A. C., Jan 1 1992, In : Journal of Cell Science. 103, 2, p. 299-307 9 p.

Research output: Contribution to journal › Article

Experimental Neoplasms

Molecular Sequence Data

Sialomucins

Neoplasm Antigens

Mucins